IC97 Is a Novel Intermediate Chain of I1 Dynein That Interacts with Tubulin and Regulates Interdoublet Sliding
IC97 Is a Novel Intermediate Chain of I1 Dynein That Interacts with Tubulin and Regulates Interdoublet Sliding
复制标题
DOI：
10.1091/mbc.e09-04-0276
复制
发表时间：
2009-07-01
影响因子：
3.3
通讯作者：
Sale, Winfield S.
中科院分区：
文献类型：
--
作者：
Wirschell, Maureen;Yang, Chun;Sale, Winfield S.
我们的目标是了解鞭毛动力蛋白，特别是衣原体内臂动力蛋白称为I1动力蛋白的装配和调节。在这里，我们专注于未表征的I1-动力蛋白IC IC 97。IC 97基因编码一种没有明显结构域的新型IC。IC 97与小鼠肺腺瘤易感性1（Las 1）蛋白-一个与肺肿瘤发生有关的候选肿瘤抑制基因-具有同源性。多个独立的生物化学测定确定了IC 97与轴丝内的α-和β-微管蛋白亚基相互作用。I1-动力蛋白装配突变体表明，IC 97与I1-动力蛋白运动复合物内的IC 138和IC 140亚基相互作用，并且IC 97是含有IC 138的调节复合物的一部分。微管滑动试验，使用轴丝含有I1动力蛋白，但没有IC 97，显示减少微管滑动速度，不救激酶抑制剂，揭示了关键作用IC 97在I1-动力蛋白的功能和控制动力蛋白驱动的运动。
Our goal is to understand the assembly and regulation of flagellar dyneins, particularly the Chlamydomonas inner arm dynein called I1 dynein. Here, we focus on the uncharacterized I1-dynein IC IC97. The IC97 gene encodes a novel IC without notable structural domains. IC97 shares homology with the murine lung adenoma susceptibility 1 (Las1) protein-a candidate tumor suppressor gene implicated in lung tumorigenesis. Multiple, independent biochemical assays determined that IC97 interacts with both alpha- and beta-tubulin subunits within the axoneme. I1-dynein assembly mutants suggest that IC97 interacts with both the IC138 and IC140 subunits within the I1-dynein motor complex and that IC97 is part of a regulatory complex that contains IC138. Microtubule sliding assays, using axonemes containing I1 dynein but devoid of IC97, show reduced microtubule sliding velocities that are not rescued by kinase inhibitors, revealing a critical role for IC97 in I1-dynein function and control of dynein-driven motility.