Enzymatic activation of the Bacillus sphaericus mosquito larvicidal toxin.
Enzymatic activation of the Bacillus sphaericus mosquito larvicidal toxin.
复制标题
球形芽孢杆菌杀幼虫毒素的酶促激活。
DOI：
10.1016/0022-2011(87)90143-1
复制
发表时间：
1987
影响因子：
3.4
通讯作者：
Shellabarger,C
中科院分区：
文献类型：
--
作者：
Davidson,EW;Bieber,AL;Meyer,M;Shellabarger,C
球形芽孢杆菌杀虫毒素经胰蛋白酶、α-凝乳胰蛋白酶和蚊子肠道匀浆活化后，对培养的蚊子细胞具有细胞毒性。高抗性埃及伊蚊幼虫的肠道提取物与高敏感性致倦库蚊的提取物一样有效地激活该毒素。激活改变了毒素的表观分子量约2-4 kDa。
Bacillus sphaericusinsecticidal toxin was activated by trypsin, α-chymotrypsin, and mosquito gut homogenates to a form which was cytotoxic to cultured mosquito cells. Gut extracts from highly resistantAedes aegyptilarvae were as effective in activating this toxin as extracts from the highly sensitive speciesCulex quinquefasciatus. Activation altered the apparent molecular weight of the toxin by ca. 2–4 kDa.