Plasmalogen mediates integration of adherens junction
Plasmalogen mediates integration of adherens junction
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缩醛磷脂介导粘附连接的整合
DOI：
10.1093/jb/mvz049
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Fujiki Yukio
中科院分区：
文献类型：
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作者：
Takahashi Takanori;Honsho Masanori;Abe Yuichi;Fujiki Yukio
醚甘油脂、缩醛磷脂存在于各种哺乳动物细胞和组织中。然而，缩醛磷脂在上皮细胞中的生理作用仍然未知。我们在此表明，含乙醇胺的缩醛磷脂（PlsEtn）的合成在MCF 7细胞（一种上皮细胞系）中是缺乏的，其中烷基-二羟基丙酮磷酸合酶（ADAPS）的表达严重降低，ADAPS是PlsEtn生物合成中的第二种酶。此外，与模拟处理的MCF 7和补充C16-烯基-PlsEtn的MCF 7细胞相比，表达ADAPS或补充含有C18-烯基残基的PlsEtn延迟了MCF 7细胞的迁移。在含有C18-烯基-PlsEtn的细胞中，E-钙粘蛋白在细胞-细胞连接处的定位高度增强。总之，这些结果表明，含有C18-烯基残基的PlsEtn在E-钙粘蛋白介导的粘附连接的完整性中起着独特的作用。
Ether glycerolipids, plasmalogens are found in various mammalian cells and tissues. However, physiological role of plasmalogens in epithelial cells remains unknown. We herein show that synthesis of ethanolamine-containing plasmalogens, plasmenylethanolamine (PlsEtn), is deficient in MCF7 cells, an epithelial cell line, with severely reduced expression of alkyl-dihydroxyacetonephosphate synthase (ADAPS), the second enzyme in the PlsEtn biosynthesis. Moreover, expression of ADAPS or supplementation of PlsEtn containing C18-alkenyl residue delays the migration of MCF7 cells as compared to that mock-treated MCF7 and C16-alkenyl-PlsEtn-supplemented MCF7 cells. Localization of E-cadherin to cell–cell junctions is highly augmented in cells containing C18-alkenyl-PlsEtn. Together, these results suggest that PlsEtn containing C18-alkenyl residue plays a distinct role in the integrity of E-cadherin-mediated adherens junction.