COMPARISON OF LASER FLUORESCENCE AND LONGITUDINAL MICRORADIOGRAPHY FOR QUANTITATIVE ASSESSMENT OF INVITRO ENAMEL CARIES
COMPARISON OF LASER FLUORESCENCE AND LONGITUDINAL MICRORADIOGRAPHY FOR QUANTITATIVE ASSESSMENT OF INVITRO ENAMEL CARIES
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DOI：
10.1159/000261446
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发表时间：
1992-07-01
期刊：
影响因子：
4.2
通讯作者：
ANGMARMANSSON, B
中科院分区：
文献类型：
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作者：
HAFSTROMBJORKMAN, U;SUNDSTROM, F;ANGMARMANSSON, B
一种新的定量，非破坏性的方法，使用激光诱导荧光（LAF）进行了比较，纵向显微放射照相术（LMR）的矿物变化的评估使用体外龋齿模型的釉质片。从人牙齿的自然光滑表面纵向切割的10个牙釉质切片，在pH循环模型中暴露于去矿化和再矿化。釉质片进行LAF和LMR测量之前和在2，4，7，和9天的脱矿。对于LAF，平均荧光辐射率在脱矿期间下降，第2天下降11%，第9天下降49%。对于LMR，相应的平均矿物质含量损失变化为0.01和0.10公斤。m2在同一时期。两种技术测量的每个单独的釉质片中的矿物质损失是强相关的，r = 0.97。所有LAF和LMR脱矿结果的斯皮尔曼等级相关系数为0.86。LAF的精密度（变异系数）为3.1%，相当于0.005 kg。m-2，LMR的重复性误差为0.02 kg . m-2，表明与LMR相比，LAF的辨别阈值较低。得出的结论是，新的，敏感的，非破坏性的LAF方法提供了进一步改进的可能性，在自然光滑的釉质表面用于体外研究的初始龋齿病变的量化。此外，它提供了潜在的原位龋研究，以及在体内早期釉质龋的诊断工具。
A new quantitative, non-destructive method using laser-induced fluorescence (LAF) was compared with longitudinal microradiography (LMR) for assessment of mineral changes in enamel slices using an in vitro caries model. Ten enamel slices, cut longitudinally from sound natural smooth surfaces of human teeth, were exposed to de- and remineralization in a pH-cycling model. The enamel slices were subjected to LAF and LMR measurements before and at 2, 4, 7, and 9 days of demineralization. For LAF, the average fluorescence radiance decreased during the demineralization period with 11 % by day 2 and 49% by day 9. For LMR, the corresponding average loss of mineral content changed with 0.01 and 0.10 kg . m-2 over the same time period. The mineral losses in each individual enamel slice measured with the two techniques were strongly correlated, r = 0.97. The Spearman rank correlation coefficient for all LAF and LMR demineralization results was 0.86. The precision (coefficient of variation) for LAF was 3.1%, corresponding to 0.005 kg . m-2, and the repeatability error for LMR was 0.02 kg . m-2, indicating a lower discrimination threshold for LAF compared to LMR. It was concluded that the new, sensitive, non-destructive LAF method provides possibilities for further improvement in the quantification of initial caries lesions in natural smooth enamel surfaces for use in in vitro studies. Furthermore, it offers potential in in situ caries studies as well as a tool in the diagnosis of early enamel caries in vivo.