Temperature-specific outcomes of cytoplasmic-nuclear interactions on egg-to-adult development time in seed beetles
Temperature-specific outcomes of cytoplasmic-nuclear interactions on egg-to-adult development time in seed beetles
复制标题
DOI：
10.1111/j.1558-5646.2007.00016.x
复制
发表时间：
2007-01-01
期刊：
影响因子：
3.3
通讯作者：
Arnqvist, Goran
中科院分区：
文献类型：
--
作者：
Dowling, Damian K.;Abiega, Katia Chavez;Arnqvist, Goran
线粒体和核基因组的整合协调了细胞能量的产生，是真核生物生命的基础。因此，强大的选择有可能塑造这两个基因组之间的相互作用。几项研究现在已经证明，细胞质和核基因之间的上位性相互作用对适合度的影响可以在“内部”和“跨”种群水平上发生。对于由核基因编码的性状来说，基因型与环境的互作是常见的，但环境异质性对部分由细胞质基因编码的性状的影响几乎没有受到关注，尽管有理由相信细胞质遗传变异的表型效应通常是环境特有的。因此，环境异质性对细胞-核适合性相互作用的结果和维持线粒体多态的重要性尚不清楚。在这里，我们评估了温度对种子甲虫(Callosobruchus Maculatus)卵到成虫发育时间的影响。我们采用了“跨种群”的设计，从五个不同的种群中寻找甲虫，并使用回交创造出不同的渗入系的正交组合，这些组合是根据它们的细胞质和核血统固定的。然后，我们分析了两种不同温度下的发育时间，发现了总体上相当大的细胞核效应，以及温度和区块特有的细胞核效应。这些结果表明，温度等环境因素确实通过有利于特定的细胞-核遗传组合来对细胞质基因进行选择，并与复杂的基因型与环境相互作用可能促进线粒体基因的多态维持的说法一致。
The integration of the mitochondrial and nuclear genomes coordinates cellular energy production and is fundamental to life among eukaryotes. Therefore, there is potential for strong selection to shape the interactions between the two genomes. Several studies have now demonstrated that epistatic interactions between cytoplasmic and nuclear genes for fitness can occur both at a "within" and "across" population level. Genotype-by-environment interactions are common for traits that are encoded by nuclear genes, but the effects of environmental heterogeneity on traits that are partly encoded by cytoplasmic genes have received little attention despite the fact that there are reasons to believe that phenotypic effects of cytoplasmic genetic variation may often be environment specific. Consequently, the importance of environmental heterogeneity to the outcomes of cyto-nuclear fitness interactions and to the maintenance of mitochondrial polymorphism is unclear. Here, we assess the influence of temperature on cyto-nuclear effects on egg-to-adult development time in seed beetles (Callosobruchus maculatus). We employed an "across-population" design, sourcing beetles from five distinct populations and using backcrossing to create orthogonal combinations of distinct introgression lines, fixed for their cytoplasmic and nuclear lineages. We then assayed development times at two different temperatures and found sizeable cyto-nuclear effects in general, as well as temperature- and block-specific cyto-nuclear effects. These results demonstrate that environmental factors such as temperature do exert selection on cytoplasmic genes by favoring specific cyto-nuclear genetic combinations, and are consistent with the suggestion that complex genotype-by-environment interactions may promote the maintenance of polymorphism in mitochondrial genes.