Antioxidant Phenolic Compounds from the Stems of Entada phaseoloides
Antioxidant Phenolic Compounds from the Stems of Entada phaseoloides
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DOI：
10.1002/cbdv.201100002
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发表时间：
2012-01-01
影响因子：
2.9
通讯作者：
Li, Xiaobo
中科院分区：
文献类型：
--
作者：
Dong, Yuqiong;Shi, Haiming;Li, Xiaobo
通过1，1-二苯基-2-苦肼基（DPPH）和2，2 '-连氮双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基清除、还原力、β-胡萝卜素漂白和超氧化物自由基清除分析，评价了恩塔达phaseoloides茎的乙醇提取物显示出强的抗氧化活性。乙醇提取物的分级显示，AcOEt级分是最活跃的，其次是H2O级分，而BuOH级分是最不活跃的。进一步的活性导向分馏研究的活性馏分导致22个化合物的分离。这些化合物的身份是建立在广泛的光谱研究的基础上。此外，通过上述五种方法对分离得到的化合物进行了抗氧化活性评价。结果表明，E.菜豆茎表现出优异的抗氧化活性，因此具有作为天然抗氧化剂来源的巨大潜力。
The EtOH extract of the stems of Entada phaseoloides displayed potent antioxidant activity when assessed by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical-scavenging, reducing power, beta-carotene-bleaching, and superoxide radical-scavenging analyses. Fractionation of the EtOH extracts showed that the AcOEt fraction is the most active, followed by the H2O fraction, while BuOH fraction was least active. Further activity-guided fractionation studies on the active fractions resulted in the isolation of 22 compounds. The identities of these compounds were established based on extensive spectroscopic studies. Furthermore, the antioxidant activities of the isolated compounds were evaluated by using the above-mentioned five assays. The results demonstrated that the EtOH extract of E. phaseoloides stems exhibits an excellent antioxidant activity and thus presents a great potential as a source of natural antioxidants.