Pulsed field gel electrophoresis. A practical guide.
Pulsed field gel electrophoresis. A practical guide.
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脉冲场凝胶电泳。
DOI:
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发表时间:
1993
期刊:
影响因子:
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通讯作者:
E. Lai
中科院分区:
文献类型:
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作者:
B. Birren;E. Lai
Introduction to Pulsed Field Gel Electrophoresis. PFGE Instrumentation. Preparation of DNA for Pulsed Field Analysis. Pouring Gels and Handling Samples. Restriction Enzyme Digestion of Large DNA Fragments in Agarose. Switch Intervals and Resolution in Pulsed Field Gels. Field Inversion Gel Electrophoresis (FIGE). Variables Which Affect Pulsed Field Gels. Southern Blot Analysis of Pulsed Field Gels. Special Applications. Trouble Shooting. Bibliography of Pulsed Field Gel References. Appendices. Chapter References.