Pulsed field gel electrophoresis. A practical guide.

Pulsed field gel electrophoresis. A practical guide.
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脉冲场凝胶电泳。

DOI:
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发表时间:
1993
期刊:
影响因子:
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通讯作者:
E. Lai
E. Lai
中科院分区:
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文献类型:
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作者:
B. Birren;E. Lai

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脉冲场凝胶电泳简介。PFGE仪器。脉冲场分析用DNA的制备浇注凝胶和处理样品。琼脂糖中大片段DNA的限制性内切酶消化。脉冲场凝胶中的开关间隔和分辨率。场反转凝胶电泳(FIGE)。影响脉冲场凝胶的变量。脉冲场凝胶的Southern印迹分析。特殊应用。故障排除。脉冲场凝胶参考书目。附录。章节参考。
Introduction to Pulsed Field Gel Electrophoresis. PFGE Instrumentation. Preparation of DNA for Pulsed Field Analysis. Pouring Gels and Handling Samples. Restriction Enzyme Digestion of Large DNA Fragments in Agarose. Switch Intervals and Resolution in Pulsed Field Gels. Field Inversion Gel Electrophoresis (FIGE). Variables Which Affect Pulsed Field Gels. Southern Blot Analysis of Pulsed Field Gels. Special Applications. Trouble Shooting. Bibliography of Pulsed Field Gel References. Appendices. Chapter References.