Mitochondrial DNA maintenance in Drosophila melanogaster.
Mitochondrial DNA maintenance in Drosophila melanogaster.
复制标题
DOI：
10.1042/bsr20211693
复制
发表时间：
2022-11-30
影响因子：
4
通讯作者：
中科院分区：
文献类型：
--
作者：
果蝇和哺乳动物的37个线粒体DNA （mtDNA）编码基因均与氧化磷酸化和线粒体内蛋白合成有关，核编码基因与mtDNA复制、转录、修复和重组有关。这一点，加上其易于遗传的可追溯性，使果蝇成为我们理解动物mtDNA维持和人类mtDNA疾病的有用模型。然而，果蝇和哺乳动物系统之间的关键区别在于动物细胞内mtDNA维持过程的多样性。在这里，我们回顾了关于果蝇mtDNA维持的已知信息，突出了需要进行更多研究的领域，并提供了这种模式生物mtDNA维持过程的组织特异性的初步计算机和体内分析的视角。我们的研究结果表明，众所周知的维持蛋白，如解旋酶Twinkle和DNA聚合酶γ的附属亚基，以及其他果蝇基因产物的新作用（或缺乏作用），甚至可能有助于阐明其他动物mtDNA的维持。我们希望为读者提供一些有趣的途径，以帮助我们的社区展示果蝇如何影响未来的mtDNA维持研究。
All 37 mitochondrial DNA (mtDNA)-encoded genes involved with oxidative phosphorylation and intramitochondrial protein synthesis, and several nuclear-encoded genes involved with mtDNA replication, transcription, repair and recombination are conserved between the fruit fly Drosophila melanogaster and mammals. This, in addition to its easy genetic tractability, has made Drosophila a useful model for our understanding of animal mtDNA maintenance and human mtDNA diseases. However, there are key differences between the Drosophila and mammalian systems that feature the diversity of mtDNA maintenance processes inside animal cells. Here, we review what is known about mtDNA maintenance in Drosophila, highlighting areas for which more research is warranted and providing a perspective preliminary in silico and in vivo analyses of the tissue specificity of mtDNA maintenance processes in this model organism. Our results suggest new roles (or the lack thereof) for well-known maintenance proteins, such as the helicase Twinkle and the accessory subunit of DNA polymerase γ, and for other Drosophila gene products that may even aid in shedding light on mtDNA maintenance in other animals. We hope to provide the reader some interesting paths that can be taken to help our community show how Drosophila may impact future mtDNA maintenance research.