Plant Stress Tolerance Requires Auxin-Sensitive Aux/IAA Transcriptional Repressors.
Plant Stress Tolerance Requires Auxin-Sensitive Aux/IAA Transcriptional Repressors.
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DOI：
10.1016/j.cub.2016.12.016
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发表时间：
2017-02-06
期刊：
影响因子：
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通讯作者：
Estelle M
中科院分区：
文献类型：
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作者：
Shani E;Salehin M;Zhang Y;Sanchez SE;Doherty C;Wang R;Mangado CC;Song L;Tal I;Pisanty O;Ecker JR;Kay SA;Pruneda-Paz J;Estelle M
Aux/IAA蛋白是植物生长素敏感的阻遏蛋白，在植物中介导多种生理和发育过程。拟南芥中有29个Aux/IAA基因表现出独特但部分重叠的表达模式（图S1 A）。虽然一些研究表明，个别Aux/IAA基因有专门的功能，该家庭的遗传分析一直受到限制的功能丧失表型的稀缺。此外，除了少数例外，我们对调节Aux/IAA表达的因素的了解是有限的。我们推测Aux/IAA基因的转录调控在调节器官发生、生长和环境响应的生长素信号通路的建立中起着核心作用。在这里，我们描述了一个屏幕的转录因子（TF）调节Aux/IAA基因。我们从38个家庭，其中包括26个成员的DREB/CBF家庭的TF。几种DREB/CBF TF直接促进响应非生物胁迫的IAA 5和IAA 19基因的转录。这些IAA基因的隐性突变导致对胁迫条件的耐受性降低，表明生长素在非生物胁迫中的作用。我们的研究结果表明，胁迫途径与生长素基因调控网络（GRN）通过Aux/IAA基因的转录相互作用。我们建议，辅助/IAA基因的功能作为枢纽，整合遗传和环境信息，以实现适当的发育或生理结果。Aux/IAA阻遏物是植物生长素反应的关键调节因子，但调节其合成的因子在很大程度上是未知的。Shani等人鉴定了调节这些基因的转录因子。DREB蛋白直接调节IAA 5和IAA 19的表达。此外，他们表明，这些Aux/IAAs所需的胁迫耐受性。
The Aux/IAA proteins are auxin-sensitive repressors that mediate diverse physiological and developmental processes in plants. There are 29 Aux/IAA genes in Arabidopsis that exhibit unique but partially overlapping patterns of expression (Figure S1A). Although some studies have suggested that individual Aux/IAA genes have specialized function, genetic analyses of the family have been limited by the scarcity of loss-of-function phenotypes. Further, with a few exceptions, our knowledge of the factors that regulate Aux/IAA expression is limited. We hypothesize that transcriptional control of Aux/IAA genes plays a central role in the establishment of the auxin-signaling pathways that regulate organogenesis, growth, and environmental response. Here we describe a screen for transcription factors (TFs) that regulate the Aux/IAA genes. We identify TFs from 38 families including 26 members of the DREB/CBF family. Several DREB/CBF TFs directly promote transcription of the IAA5 and IAA19 genes in response to abiotic stress. Recessive mutations in these IAA genes result in decreased tolerance to stress conditions demonstrating a role for auxin in abiotic stress. Our results demonstrate that stress pathways interact with the auxin gene regulatory network (GRN) through transcription of the Aux/IAA genes. We propose that the Aux/IAA genes function as hubs that integrate genetic and environmental information to achieve the appropriate developmental or physiological outcome. The Aux/IAA repressors are key regulators of auxin response in plants but the factors that regulate their synthesis are largely unknown. Shani et al. identify transcription factors that regulate these genes. The DREB proteins directly regulate expression of IAA5 and IAA19. Further they show that these Aux/IAAs are required for stress tolerance.