In vivo gene expression of Pseudomonas putida KT2440 in the rhizosphere of different plants
In vivo gene expression of Pseudomonas putida KT2440 in the rhizosphere of different plants
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DOI：
10.1111/1751-7915.12037
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发表时间：
2013-05-01
影响因子：
5.7
通讯作者：
Ramos, Juan-Luis
中科院分区：
文献类型：
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作者：
Fernandez, Matilde;Conde, Susana;Ramos, Juan-Luis
恶臭假单胞菌 KT2440 能够在多种植物的根际定殖，细胞密度可以达到 10(5)-10(6) cfu g 土壤(-1) 范围。使用IVET技术，我们研究了哪些KT2440基因在四种不同植物的根际表达：松树、柏树、常绿橡树和迷迭香。我们鉴定了 39 个不同的转录融合体，其中包含优先在根际表达的注释基因的启动子。其中 6 个融合体在所有测试植物类型的根际中表达，11 个在不止一种植物中表达，其余 22 个融合体仅在一种植物中表达。发现另外 40 个融合体可能对应于编码功能未知的反义 RNA 的启动子，其中一些是从所有植物根际中回收的细菌中作为融合体分离出来的，而另一些则是一种或几种植物特有的。本研究获得的结果表明，KT2440 在根际感知植物特异性信号，并且这些信号和随后的基因表达与细菌在该生态位中的成功建立有关。
Pseudomonas putida KT2440 has the ability to colonize the rhizosphere of a wide range of plants and can reach cell densities in the range of 10(5)-10(6) cfu g soil(-1). Using the IVET technology we investigated which KT2440 genes were expressed in the rhizosphere of four different plants: pine, cypress, evergreen oak and rosemary. We identified 39 different transcriptional fusions containing the promoters of annotated genes that were preferentially expressed in the rhizosphere. Six of them were expressed in the rhizosphere of all the plant types tested, 11 were expressed in more than one plant and the remaining 22 fusions were found to be expressed in only one type of plant. Another 40 fusions were found to correspond to likely promoters that encode antisense RNAs of unknown function, some of which were isolated as fusions from the bacteria recovered in the rhizosphere from all of the plants, while others were specific to one or several of the plants. The results obtained in this study suggest that plant-specific signals are sensed by KT2440 in the rhizosphere and that the signals and consequent gene expression are related to the bacteria's successful establishment in this niche.