Agroinoculation shows Tobacco leaf curl Yunnan virus is a monopartite begomovirus
Agroinoculation shows Tobacco leaf curl Yunnan virus is a monopartite begomovirus
复制标题
农杆菌接种表明云南卷叶病毒为单部分begomovirus
DOI：
10.1007/s10658-006-9021-8
复制
发表时间：
2006-08-01
影响因子：
1.8
通讯作者：
Zhou, Xueping
中科院分区：
文献类型：
--
作者：
Xie, Yan;Jiang, Tong;Zhou, Xueping
结果表明，云南烟草曲叶病毒（Tobacco leaf curl Yunnan virus，TbLCYNV）的15个分离物中，只有2个分离物与卫星DNA β分子相关。为了研究该病毒的侵染性，将TbLCYNV分离物Y143的侵染性克隆（TbLCYNV-Y143）接种于本氏烟草（Nicotianabenthamiana）和烟草粉虱（Nicotianabenthamiana），并通过烟粉虱传播，获得了TbLCYNV-Y143的侵染性克隆。矮牵牛、矮牵牛和N.烟草。单独的TbLCYNV-Y143能够在这些植物中诱导严重的向上叶片卷曲、脉增厚或矮化症状。TbLCYNV-Y143与其他菜豆花叶病毒相关的DNA β分子的共接种在这些植物上诱导了相似的症状类型。这表明TbLCYNV是一种单分裂的菜豆花叶病毒。只有两个分离株TbLCYNV与DNA β分子的结果的相关性进行了讨论。
We demonstrated that only 2 out of 15 isolates of Tobacco leaf curl Yunnan virus (TbLCYNV) were associated with the satellite DNA beta molecules. To investigate the infectivity of this virus, an infectious clone of TbLCYNV isolate Y143 (TbLCYNV-Y143) was agroinoculated or whitefly transmitted into Nicotiana benthamiana, N. glutinasa, Petunia hybrida and N. tabacum. TbLCYNV-Y143 alone was able to induce severe upward leaf curling, vein thickening or stunt symptoms in these plants. Co-inoculation of TbLCYNV-Y143 with DNA beta molecules associated with other begomoviruses induced similar symptom types on these plants. This indicates that TbLCYNV is a monopartite begomovirus. The relevance of results that only two isolates of TbLCYNV were associated with DNA beta molecules is discussed.