Haliea mediterranea sp nov., a marine gammaproteobacterium
Haliea mediterranea sp nov., a marine gammaproteobacterium
复制标题
DOI：
10.1099/ijs.0.017061-0
复制
发表时间：
2010-08-01
影响因子：
2.8
通讯作者：
Pujalte, Maria J.
中科院分区：
文献类型：
--
作者：
Lucena, Teresa;Pascual, Javier;Pujalte, Maria J.
7SM29(T)菌株是从西班牙卡斯特隆海水中分离到的一株好氧海洋伽马蛋白杆菌，经经典表型分析、化学分类学和16S rRNA基因序列分析鉴定为7SM29(T)。结果表明，7SM29(T)菌株与Haliea属的菌株和斜纹拟杆菌KT71(T)有较近的亲缘关系，在GammaProteteria的NOR5/OM60沼泽区内形成了一个属水平的类群。菌株7SM29(T)是一根短的、可移动的杆状物，有一簇三极鞭毛。该菌株生长在海洋琼脂上，形成淡黄色的菌落。菌株75M29(T)生长需要氯化钠，将硝酸盐还原为亚硝酸盐，降解几种聚合物，并显示出对有机酸和氨基酸的偏好，而不是碳水化合物作为碳源。菌株7SM29(T)含有唯一的呼吸对苯二酚Q-8。DNA G+C含量为62.1mol%。磷脂酰甘油、二磷脂酰甘油和磷脂酰乙醇胺是主要的极性脂类。细胞脂肪酸主要为不饱和C-16-C-18化合物。在广泛的表型和系统发育分析的基础上，该菌株代表了Haliea属的一个新种，命名为Haliea Mediterranea sp.11月是提议的。模式株为7SM29(T)(=CECT7447(T)=DSM 21924(T))。
Strain 7SM29(T), an aerobic marine gammaproteobacterium isolated from seawater from Castellon, Spain, was characterized by classical phenotyping, chemotaxonomy and 16S rRNA gene sequence analysis. Strain 7SM29(T) was found to be closely related to strains in the genus Haliea and to Congregibacter litoralis KT71(T), with which a genus-level cluster was formed within the NOR5/OM60 Glade of the Gammaproteobacteria. Strain 7SM29(T) was a short, motile rod with a tuft of three polar flagella. The strain grew on marine agar and formed pale-yellow colonies. Strain 75M29(T) required NaCl for growth, reduced nitrate to nitrite, degraded several polymers and showed a preference for organic acids and amino acids over carbohydrates as carbon and energy sources. Strain 7SM29(T) contained Q-8 as the sole respiratory quinone. The DNA G+C content was 62.1 mol%. Phosphatidylglycerol, diphosphatidylglycerol and phosphatidylethanolamine were the major polar lipids. The major cellular fatty acids were unsaturated C-16-C-18 compounds. On the basis of extensive phenotypic and phylogenetic comparative analysis, it is concluded that the strain represents a novel species of the genus Haliea, for which the name Haliea mediterranea sp. nov. is proposed. The type strain is 7SM29(T) (=CECT 7447(T) =DSM 21924(T)).