USE OF CAFFEINE METABOLITE RATIOS TO EXPLORE CYP1A2 AND XANTHINE-OXIDASE ACTIVITIES
USE OF CAFFEINE METABOLITE RATIOS TO EXPLORE CYP1A2 AND XANTHINE-OXIDASE ACTIVITIES
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DOI：
10.1038/clpt.1991.176
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发表时间：
1991-11-01
影响因子：
6.7
通讯作者：
TANG, BK
中科院分区：
文献类型：
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作者：
KALOW, W;TANG, BK
以咖啡因为代谢探针，对健康受试者的两种酶（推测为CYP1A2（一种诱导型细胞色素P450）和黄嘌呤氧化酶）的活性进行了筛选。一项纵向研究显示，咖啡因剂量、乙醇摄入量和一天中的时间对CYP1A2指数有适度的影响，对黄嘌呤氧化酶指数没有任何影响。未考虑的个体内变异系数为5.0%的黄嘌呤氧化酶和17.2%的CYP1A2指数。在一项人群研究中，两项指标均呈对数正态分布，大多数受试者的CYP1A2值范围为6.3倍，但黄嘌呤氧化酶仅为1.7倍。CYP1A2指数在使用口服避孕药的女性中下降了33%，在吸烟者中大幅增加。无论是CYP1A2，也没有黄嘌呤氧化酶指数不同的志愿者之间的中国和欧洲的提取。178名受试者中有4名显示出无法解释的低黄嘌呤氧化酶值（即，值低于平均值几个标准偏差）。
Caffeine was used as a metabolic probe to screen healthy subjects for their activities of two enzymes, deduced to be CYP1A2 (an inducible cytochrome P450) and xanthine oxidase. A longitudinal study revealed modest effects of caffeine dose, ethanol intake, and time-of-day on the CYP1A2 index, without any effect on the xanthine oxidase index. The coefficients of intraindividual variation not accounted for were 5.0% for the xanthine oxidase and 17.2% for the CYP1A2 index. In a population study, both indexes showed a log normal distribution, with CYP1A2 values of most subjects covering a 6.3-fold range but only a 1.7-fold range with xanthine oxidase. The CYP1A2 index was 33% decreased in women who used oral contraceptives and substantially increased in cigarette smokers. Neither the CYP1A2 nor the xanthine oxidase index differed between volunteers of Chinese and European extraction. Four of 178 subjects showed unexplained low xanthine oxidase values (i.e., values several standard deviations below the mean).