The SPOT synthesis technique - Synthetic peptide arrays on membrane supports - principles and applications
The SPOT synthesis technique - Synthetic peptide arrays on membrane supports - principles and applications
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DOI：
10.1016/s0022-1759(02)00137-0
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发表时间：
2002-09-01
影响因子：
2.2
通讯作者：
Frank, R
中科院分区：
文献类型：
--
作者：
Frank, R
首次于1990年在西班牙巴塞罗那举行的第21届欧洲肽研讨会上提出[Frank，R.，Guler，S.，克劳斯，S.，Lindenmaier，W.，1991.在膜片上方便快速地“点合成”大量肽。在：Giralt，E.，Andreu，D.（编辑）Peptides 1990，Proc.21st Eur.肽合成ESCOM，Leiden，第151页]，SPOT合成方法为合成和随后筛选排列在平面纤维素载体上的大量合成肽以及其它有机化合物提供了无数的机会[Tetrahedron 48（1992）9217]。早在1991年，剑桥生物化学研究所（CRB，联合王国）就已提供了一种用于手工合成SPOT的商业试剂盒，1993年，德国的ABIMED分析技术公司推出了一种半自动SPOT合成仪ASP 222。两者都使许多研究实验室，甚至那些没有化学经验或装备的实验室，都可以使用这种技术。虽然SPOT合成不像例如Affymax PCR技术那样令人印象深刻，但它满足了类似的要求，具有可靠和容易的实验程序、廉价的设备需求和高度灵活的阵列和文库格式的优点。该方法允许快速和高度平行合成大量的肽和肽混合物（池），包括各种各样的非天然结构单元，以及越来越多的其他有机化合物。其他优点涉及易于适应广泛的测定和筛选方法，例如结合、酶和细胞测定，由于膜支持物的特殊性质，其允许原位筛选化学文库。因此，通过SPOT技术制备的肽阵列成为研究分子识别的许多方面的非常流行的工具，特别是在分子免疫学领域。(C)2002 Elsevier Science B.V.保留所有权利。
Presented first in 1990 at the 21st European Peptide Symposium in Barcelona, Spain [Frank, R., Guler, S., Krause, S., Lindenmaier, W., 1991. Facile and rapid 'spot synthesis' of large numbers of peptides on membrane sheets. In: Giralt, E., Andreu, D. (Eds.) Peptides 1990, Proc. 21st Eur. Peptide Symp. ESCOM, Leiden, p. 151.], the SPOT synthesis method opened up countless opportunities to synthesise and subsequently screen large numbers of synthetic peptides as well as other organic compounds arrayed on a planar cellulose support [Tetrahedron 48 (1992) 9217]. Already in 1991, a commercial kit for manual SPOT synthesis became available through Cambridge Research Biochemicals (CRB, UK), and in 1993, a semi-automated SPOT-synthesiser, the ASP222, was launched by ABIMED Analysen-Technik, Germany. Both made the technique available to many research laboratories, even those not experienced in or equipped for chemistry. Although SPOT synthesis is not as impressively miniaturised as, e.g. the Affymax photolithographic technique [Science 251 (1991) 767], it fulfils similar demands with the advantage of a reliable and easy experimental procedure, inexpensive equipment needs and a highly flexible array and library formatting. The method permits rapid and highly parallel synthesis of huge numbers of peptides and peptide mixtures (pools) including a large variety of unnatural building blocks, as well as a growing range of other organic compounds. Further advantages are related to the easy adaptability to a wide range of assay and screening methods such as binding, enzymatic and cellular assays, which allow in situ screening of chemical libraries due to the special properties of the membrane supports. Therefore, peptide arrays prepared by the SPOT technique became quite popular tools for studying numerous aspects of molecular recognition, particularly in the field of molecular immunology. (C) 2002 Elsevier Science B.V. All rights reserved.