High-Performance Liquid Chromatographic Purification of Oligomeric Procyanidins, Trimers up to Nonamers, Derived from the Bark of Jatoba (Hymenaea courbaril)
High-Performance Liquid Chromatographic Purification of Oligomeric Procyanidins, Trimers up to Nonamers, Derived from the Bark of Jatoba (Hymenaea courbaril)
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对源自 Jatoba (Hymenaea courbaril) 树皮的低聚原花青素、三聚体至九聚体进行高效液相色谱纯化
DOI：
10.1271/bbb.80747
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Y. Konishi
中科院分区：
文献类型：
--
作者：
K. Sasaki;Y. Matsukura;Kumiko Shijima;M. Miyake;Daisuke Fujiwara;Y. Konishi
利用最近发展起来的色谱分离方法，从贾托巴（Hymenaea courbaril）树皮中有效地纯化了不同聚合度（最高可达九聚体）的原花青素低聚物。纯化依赖于在填充柱中原花青素的酚羟基与聚乙二醇（PEG）涂层树脂之间的氢键相互作用。通过电喷雾质谱（ESI-MS）鉴定了各个原花青素，并通过硫解降解分析进行验证。我们的研究结果表明，贾托巴树皮含有大量的原花青素从单体到九聚体或更高的聚合物组成的B型连接单元（黄烷-3-醇单元连接通过C-4至C-8（或C-6））的表儿茶素（EC）没有没食子酸酯。
Procyanidin oligomers with different degrees of polymerization (up to nonamers) were efficiently purified from the bark of Jatoba (Hymenaea courbaril) by using a recently developed chromatographic separation method. Purification relied on a hydrogen bonding interaction between phenolic hydroxyl groups of the procyanidins and polyethylene glycol (PEG)-coated resin in a packed column. The individual procyanidins were identified by using electrospray ionization mass spectrometry (ESI-MS) and verified by a thiolytic degradation analysis. Our results demonstrate that Jatoba bark contained a large amount of procyanidins from monomer to nonamers or higher polymers composed of only B-type linked units (flavan-3-ol units linked through C-4 to C-8 (or C-6)) of epicatechin (EC) without gallate esters.
影响因子：
6.1
作者：
Alan W. Taylor;E. Barofsky;J. Kennedy;M. Deinzer
通讯作者：
Alan W. Taylor;E. Barofsky;J. Kennedy;M. Deinzer
影响因子：
4.7
作者：
Jifu Zhao;Jiannong Wang;Yingjie Chen;R. Agarwal
通讯作者：
Jifu Zhao;Jiannong Wang;Yingjie Chen;R. Agarwal