ENTEROBACTER-SAKAZAKII - A NEW SPECIES OF ENTEROBACTERIACEAE ISOLATED FROM CLINICAL SPECIMENS
ENTEROBACTER-SAKAZAKII - A NEW SPECIES OF ENTEROBACTERIACEAE ISOLATED FROM CLINICAL SPECIMENS
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DOI：
10.1099/00207713-30-3-569
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发表时间：
1980-01-01
期刊：
影响因子：
--
通讯作者：
BRENNER, DJ
中科院分区：
文献类型：
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作者：
FARMER, JJ;ASBURY, MA;BRENNER, DJ
阪崎肠杆菌是以前所知的“黄色素阴沟肠杆菌”的名称。该种的模式菌株(正型)为atcc 29544。建议对这种生物体的分类进行更改是基于E。泄殖腔和E。Skazakiiiin脱氧核糖核酸(DNA)-DNA杂交、生化反应、色素产生和抗生素敏感性。通过DNA杂交，阪崎肠杆菌与趾的亲缘关系约占50%。阴沟杆菌、多样性柠檬酸杆菌(生物型b型)和苦味柠檬酸杆菌(生物型a型)。新物种被归入肠杆菌而不是柠檬菌，因为它的表型和DNA与脚趾更相似。阴沟杆菌是肠杆菌属的模式种，DNA杂交显示它与柠檬酸杆菌的模式种弗伦迪柠檬酸杆菌只有41%的亲缘关系。SAKAKAKI的生化反应与E。阴沟肠杆菌D-山梨醇阴性，胞外脱氧核糖核酸酶2~7天呈阳性，并产生黄色菌落。阪崎肠杆菌在氨苄西林和头孢菌素抗生素纸片周围有较大的抑制带，这也有助于将其与Me区分开来。泄殖腔。在肠道细菌学常用的非选择性(但有差异)的培养基上，其培养效率降低，但在抑制性较强的肠道培养基上，其培养效率降低。它已从人类临床标本中分离出来，如痰、粪便和伤口，在这些地方它可能只是一个殖民者，没有临床意义。然而，它也是一种记录在案的新生儿脑膜炎的原因，尽管很罕见。其他来源包括食物、医生的听诊器和一个未接种的血液培养瓶。
Enterobacter sakazakiiis the name proposed for the organism previously known as “yellow-pigmentedEnterobacter cloacae.” The type strain (holotype) of this species is ATCC 29544. The proposed change in the classification of this organism is based on differences betweenE. cloacaeandE. sakazakiiin deoxyribonucleic acid (DNA)-DNA hybridization, biochemical reactions, pigment production, and antibiotic susceptibility. By DNA hybridization,E. sakazakiiwas about 50% related toE. cloacae, Citrobacter diversus(“Citrobacter intermedius” biotype b), and “Citrobacter amalonaticus” (“Citrobacter intermedius” biotype a). The new species was placed inEnterobacterrather thanCitrobacterbecause of its closer phenotypic and DNA similarity toE. cloacae, the type species of the genusEnterobacter, and because it was only 41% related by DNA hybridization toCitrobacter freundii, the type species ofCitrobacter. E. sakazakiihad biochemical reactions very similar to those ofE. cloacaebut was d-sorbitol negative and positive for extracellular deoxyribonuclease at 2 to 7 days and produced yellow-pigmented colonies.E. sakazakiihad larger zones of inhibition around ampicillin and cephalothin antibiotic disks, which also helps to differentiate it fromE. cloacae. E. sakazakiigrew on the nonselective (but differential) plating media commonly used in enteric bacteriology, but its plating efficiency was reduced on more inhibitory enteric plating media. It has been isolated from human clinical specimens such as sputum, feces, and wounds, where it is probably only a colonizer and not clinically significant. However, it is also a documented, although rare, cause of neonatal meningitis. Other sources have included food, a physician’s stethoscope, and an uninoculated blood culture bottle.