AMINO-ACID UTILIZATION PATTERNS IN CLOSTRIDIAL TAXONOMY
AMINO-ACID UTILIZATION PATTERNS IN CLOSTRIDIAL TAXONOMY
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DOI：
10.1007/bf00446812
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发表时间：
1979-01-01
影响因子：
2.8
通讯作者：
HILTON, MG
中科院分区：
文献类型：
--
作者：
ELSDEN, SR;HILTON, MG
用于二甲氨基萘磺酰基氨基酸色谱分离的聚酰胺层技术用于研究在由作为氨基酸来源的酪蛋白酸水解产物和作为生长因子来源的少量酵母提取物和胰蛋白酶组成的培养基中生长的蛋白水解梭菌培养物代谢的氨基酸。生长后培养基的色谱图显示使用了哪些氨基酸以及产生了哪些新氨基酸。 F 型肉毒梭菌（蛋白水解）、高尼梭菌、曼根诺梭菌和腐败梭菌将脯氨酸还原为 5-氨基戊酸并产生 2-氨基丁酸，这些特性与产孢梭菌和斯氏梭菌相同。 G型肉毒杆菌和亚末端肉毒杆菌使用甘氨酸、赖氨酸、丝氨酸和精氨酸，但与斯氏肉毒杆菌不同，它们既不将脯氨酸还原为5-氨基戊酸，也不产生2-氨基丁酸。两种生物体分别将苯丙氨酸、酪氨酸和色氨酸氧化为苯乙酸、对羟基苯乙酸和吲哚乙酸。 C. lituseburense 和 C. scatologenes 使用丝氨酸、苏氨酸和精氨酸并产生 2-氨基丁酸和鸟氨酸。 C. lentoputrescens、C. limosum 和 C.malenominatum 通过使用谷氨酸和酪氨酸而与 C. tetanomorphum 相似。色谱图总是显示生物体所属的生理组，并且在某些情况下显示该物种的特征。
The polyamide layer technique for chromatographic separation of dimethylaminonaphthalene sulfonyl amino acids was used to study the amino acids metabolized by cultures of proteolytic clostridia growing in a medium consisting of an acid hydrolysate of casein as a source of amino acids and small amounts of yeast extract and trypticase as sources of growth factors. Chromatograms of the media after growth showed which amino acids were used and which new amino acids were produced. Clostridium botulinum type F (proteolytic), C. ghoni, C. mangenoti and C. putrificum reduced proline to 5-aminovaleric acid and produced 2-aminobutyric acid, properties they shared with C. sporogenes and C. sticklandii. C. botulinum type G and C. subterminale used glycine, lysine, serine and arginine but, in contrast to C. sticklandii, they neither reduced proline to 5-aminovaleric acid nor produced 2-aminobutyric acid. Both organisms oxidized phenylalanine, tyrosine and tryptophan to phenylacetic acid, p-hydroxyphenyl acetic acid and indole acetic acid, respectively. C. lituseburense and C. scatologenes used serine, threonine and arginine and produced 2-amino butyric acid and ornithine. C. lentoputrescens, C. limosum and C. malenominatum resembled C. tetanomorphum by using glutamic acid and tyrosine. The chromatograms always showed the physiological group to which an organism belonged and in some cases were characteristic of the species.