A microfluidic finger-actuated blood lysate preparation device enabled by rapid acoustofluidic mixing
A microfluidic finger-actuated blood lysate preparation device enabled by rapid acoustofluidic mixing
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DOI：
10.1101/2022.10.16.512425
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发表时间：
2022-10
期刊：
影响因子：
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通讯作者：
Md Ehtashamul Haque;A. Conde;W. Macpherson;Stephen R. Knight;R. Carter;M. Kersaudy-Kerhoas
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文献类型：
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作者：
Md Ehtashamul Haque;A. Conde;W. Macpherson;Stephen R. Knight;R. Carter;M. Kersaudy-Kerhoas
对于许多基于血液的诊断测试，包括预防性药物分析和疟疾分析，红细胞必须在分析工作流程中使用之前进行有效裂解。我们报告了一种手指驱动的血液裂解液制备装置，该装置利用了先前报道的声流控微混合器模块。该集成设备包括一系列创新，从样品接口到激光雕刻表面上的水泡集成，以及大体积（130 μL）单冲程手动泵，可用于其他低成本的基于微流体的护理点设备。声学混合器在高粘性流体（包括红细胞体积分数高达65%的全血）中的适应性得到了证明。与裂解缓冲液一起使用时，微混合器单元还显示在30秒内裂解手指刺破（20 μL）血液样本，并在10个供体样本中进行基准测试。最后，我们演示了全集成器件的易用性。便宜，模块化，但可靠，手指驱动的微流控功能可以用最少的资源开辟诊断发展的机会。
For many blood-based diagnostic tests, including prophylactic drug analysis and malaria assays, red blood cells must be lysed effectively prior to their use in an analytical workflow. We report on a finger-actuated blood lysate preparation device, which utilises a previously reported acoustofluidic micromixer module. The integrated device includes a range of innovations from a sample interface, to the integration of blisters on a laser engraved surface and a large volume (130 μL) one-stroke manual pump which could be useful in other low-cost microfluidic-based point-of-care devices. The adaptability of the acoustic mixer is demonstrated on highly viscous fluids, including whole blood, with up to 65% percent volume fraction of red blood cells. Used in conjunction with a lysis buffer, the micromixer unit is also shown to lyse a finger-prick (20 μL) blood sample in 30 seconds and benchmarked across ten donor samples. Finally, we demonstrate the ease of use of the fully integrated device. Cheap, modular, but reliable, finger-actuated microfluidic functions could open up opportunities for the development of diagnostics with minimal resources.