Sperm storage and motility in the ovary of the marine sculpin Alcichthys alcicornis (Teleostei: Scorpaeniformes), with internal gametic association.
Sperm storage and motility in the ovary of the marine sculpin Alcichthys alcicornis (Teleostei: Scorpaeniformes), with internal gametic association.
复制标题
海洋杜父鱼 Alcichthys alcicornis（Teleostei：Scorpaeniformes）卵巢中的精子储存和运动，具有内部配子关联。
DOI：
10.1002/jez.1150
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
K. Takano
中科院分区：
文献类型：
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作者：
Y. Koya;H. Munehara;K. Takano
Elcichthys alcicornis是一种海生硬骨鱼，具有一种独特的繁殖模式，称为内部配子联想，在这种模式下，通过交配进入卵巢的精子进入卵巢腔内排出的卵子的珠孔通道，但真正的精卵融合要等到卵子被释放到海水中才能发生。人们还知道，这种鱼是一种多产卵的鱼，每隔几天产卵一次，持续一个月，在产卵季节开始时引入卵巢的精子在整个时期保持受精性。为了阐明精子的受精能力是如何在体内维持的，对精子的超微结构、与卵巢中精子储存有关的形态特征以及精子的运动特性进行了研究。成熟精子通常具有正常形态的硬骨鱼精子，没有顶体结构。然而，发现中段相对拉长，并有许多小尺寸线粒体的紧密聚集。在整个产卵季节，卵巢内精子一直漂浮在卵巢液中。精子在含钠离子的等渗和弱碱性溶液中表现出较高的活力，与卵巢液相似。精子在人工卵巢液中持续运动7~14天。综合考虑这些结果，人们认为卵巢内精子在整个产卵季节都在移动，这是因为许多线粒体产生了充足的能量。
Elkhorn sculpin, Alcichthys alcicornis, is a marine teleost with a unique reproductive mode called "internal gametic association," in which sperm introduced into the ovary by copulation enter the micropylar canal of ovulated eggs in the ovarian cavity, but actual sperm-egg fusion does not occur until the eggs have been released into sea water. It is also known that this fish is a multiple spawner, which spawns at intervals of a few days for one month, and the sperm introduced into the ovary at the beginning of the spawning season retain their fertilizability for the entire period. To clarify how the fertilizability of sperm is maintained internally, the ultrastructure of sperm, the morphological characteristics related to sperm storage in the ovary, and the characteristics of sperm motility were investigated. Mature sperm generally have the normal form of teleost sperm, devoid of acrosomal structures. However, it was found that the midpiece is comparatively elongated and has a compact aggregation of many small-size mitochondria. The intraovarian sperm remained floating in the ovarian fluid throughout the spawning season. The sperm showed high motility in isotonic and weak alkaline solution, containing sodium ions, which was similar to the ovarian fluid of this fish. Sperm continued to move in artificial ovarian fluid for 7-14 days. Considering these results together, it is thought that the intraovarian sperm move throughout the spawning season due to the plentiful energy generated by the many mitochondria.