Self-Assembly of Large DNA Origami with Custom-Designed Scaffolds
Self-Assembly of Large DNA Origami with Custom-Designed Scaffolds
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使用定制设计的支架自组装大型 DNA 折纸
DOI：
10.1021/acsami.8b09222
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发表时间：
2018-07-25
影响因子：
9.5
通讯作者：
Li, Zhe
中科院分区：
文献类型：
--
作者：
Chen, Xiaoxing;Wang, Qian;Li, Zhe
DNA“折纸”作为DNA自组装的一个里程碑，在许多领域都有着广泛的应用。然而，由于长单链DNA（ssDNA）的稀缺性，限制了DNA折纸纳米结构的大小和序列，这反过来又阻碍了进一步的发展。在这项研究中，我们提出了一个强大的战略，以生产长的环状ssDNA支架链定制的长度和序列。然后将这些ssDNA产物用作构建各种DNA折纸纳米结构的支架。这种可扩展的方法以低成本、高纯度和高产率生产ssDNA，这可以使得能够生产用于各种应用的定制设计的DNA折纸。
As a milestone in DNA self-assembly, DNA origami has demonstrated powerful applications in many fields. However, the scarce availability of long single-stranded DNA (ssDNA) limits the size and sequences of DNA origami nanostructures, which in turn impedes the further development. In this study, we present a robust strategy to produce long circular ssDNA scaffold strands with custom-tailored lengths and sequences. These ssDNA products were then used as scaffolds for constructing various DNA origami nanostructures. This scalable method produces ssDNA at low cost with high purity and high yield, which can enable production of custom-designed DNA origami for various applications.