Microarray profiling of colorectal cancer in Bangladeshi patients
Microarray profiling of colorectal cancer in Bangladeshi patients
复制标题
DOI：
10.1111/j.1463-1318.2005.00818.x
复制
发表时间：
2005-11-01
影响因子：
3.4
通讯作者：
Dorudi, S
中科院分区：
文献类型：
--
作者：
Ahmed, S;Banerjea, A;Dorudi, S
目的我们对1998年4月至2002年3月间在皇家伦敦医院就诊的所有结直肠癌病例进行了回顾性分析，以确定孟加拉国和非孟加拉国患者在临床表现和预后方面的差异。DNA微阵列被用来解释这两组之间的任何潜在的遗传差异，可以解释不同的phenotypes.Materials和方法，我们检查了在我们的机构的结肠直肠数据库。微阵列的配置文件，使用AffyphineHU 133 A基因芯片（TM）（圣克拉拉，CA美国），从10名孟加拉国患者和年龄，性别和阶段匹配组的10名非孟加拉国patients.Results三百六十三例已治疗结直肠癌在皇家伦敦医院。18例（5%）患者为孟加拉国人。患病率为27/100 000，而非孟加拉国人为342/100 000。18例孟加拉国患者中有11例（61%）年龄在40岁以下，4例（22%）患者出现局部晚期或转移性疾病。相比之下，39/345（11%）的非孟加拉国患者表现为晚期疾病。孟加拉国患者中没有一人有阳性家族史。结论孟加拉国人结直肠癌较非孟加拉国人少见，孟加拉国人结直肠癌与非孟加拉国人结直肠癌相比，差异表达基因数为1203个（P < 0.05）。这种癌症出现在更年轻的患者和更先进的阶段。在这个种族社区内没有阳性家族史，因此癌症是散发性的。然而，微阵列分析能够描绘这两组之间的不同基因表达。因此，应该有一个低门槛的调查年轻的孟加拉国结直肠肿瘤患者的症状和任何未来的国家筛查计划应允许种族差异。
Objective We have carried out a retrospective analysis of all cases of colorectal cancer at the Royal London Hospital between April 1998 and March 2002 and determined the differences in presentation and outcome between Bangladeshi and Non-Bangladeshi patients. DNA microarrays were used to explain any potential genetic differences between these two groups that may explain the different phenotypes.Materials and methods We examined the colorectal database at our institution. Microarray profiles, using Affymetrix HU133A Genechips (TM) (Santa Clara, CA USA) were obtained from 10 Bangladeshi patients and an age-, sex- and stage-matched group of 10 Non- Bangladeshi patients.Results Three hundred and sixty-three patients have been treated for colorectal cancer at the Royal London Hospital. Eighteen (5%) patients were of Bangladeshi origin. The prevalence was 27/100 000 compared to 342/100 000 of the Non-Bangladeshi population. Eleven (61%) of 18 Bangladeshi patients were under the age of 40 and 4 (22%) patients presented with locally advanced or metastatic disease. In comparison 39/345 (11%) of non-Bangladeshi patients presented with advanced disease. None of the Bangladeshi patients gave a positive family history. Microarray profiling between these two groups demonstrated 1203 differentially expressed genes (P < 0.05).Conclusion Colorectal cancer is uncommon in the Bangladeshi patients compared to the non-Bangladeshi population. This cancer presents in younger patients and at a more advanced stage. There is no positive family history within this ethnic community and therefore the cancers are sporadic. However, microarray profiling is able to delineate different gene expression between these two groups. Therefore, there should be a low threshold for investigating young Bangladeshi patients with symptoms of colorectal neoplasia and any future national screening programme should allow for ethnic variation.