Impact of contusion injury on intramuscular emm1 group a streptococcus infection and lymphatic spread.
Impact of contusion injury on intramuscular emm1 group a streptococcus infection and lymphatic spread.
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挫伤损伤对肌内EMM1 A组链球菌感染和淋巴扩散的影响。
DOI：
10.1080/21505594.2018.1482180
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发表时间：
2018
期刊：
影响因子：
5.2
通讯作者：
Sriskandan S
中科院分区：
文献类型：
--
作者：
Lamb LE;Siggins MK;Scudamore C;Macdonald W;Turner CE;Lynskey NN;Tan LKK;Sriskandan S
侵袭性A组链球菌（iGAS）经常与emm1分离株相关，伴随的死亡率约为20%。病例偶尔出现在以前健康的个体中，有上呼吸道感染、软组织挫伤和无明显入口的病史。使用新的挫伤小鼠模型，我们确定了挫伤对iGAS细菌负荷和表型的影响。经校准的轻度钝挫伤并不提供在全身性GAS菌血症后可检测到的GAS启动或接种的病灶，而是在挫伤的同时和部位接种GAS后增强GAS向局部引流淋巴结的迁移。向淋巴结迁移的增加与粘液细菌的出现相关，尽管不是粘液细菌特异性的。在一项研究中，粘液样集落显示出囊状透明质酸的显著增加，其与covRS或rocA突变无关，但与囊状合成基因座hasABC的启动子缺失有关，导致菌株对淋巴结迁移的适应性增加。总之，在使用的轻度挫伤模型中，我们不能通过GAS检测到肌肉的接种。挫伤促进细菌转运至局部淋巴结。挫伤相关的细菌淋巴迁移的后果可能会有所不同，这取决于所选择的病原体和毒力性状。
Invasive group A Streptococcus (iGAS) is frequently associated with emm1 isolates, with an attendant mortality of around 20%. Cases occasionally arise in previously healthy individuals with a history of upper respiratory tract infection, soft tissue contusion, and no obvious portal of entry. Using a new murine model of contusion, we determined the impact of contusion on iGAS bacterial burden and phenotype. Calibrated mild blunt contusion did not provide a focus for initiation or seeding of GAS that was detectable following systemic GAS bacteremia, but instead enhanced GAS migration to the local draining lymph node following GAS inoculation at the same time and site of contusion. Increased migration to lymph node was associated with emergence of mucoid bacteria, although was not specific to mucoid bacteria. In one study, mucoid colonies demonstrated a significant increase in capsular hyaluronan that was not linked to a covRS or rocA mutation, but to a deletion in the promoter of the capsule synthesis locus, hasABC, resulting in a strain with increased fitness for lymph node migration. In summary, in the mild contusion model used, we could not detect seeding of muscle by GAS. Contusion promoted bacterial transit to the local lymph node. The consequences of contusion-associated bacterial lymphatic migration may vary depending on the pathogen and virulence traits selected.
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