Fatigue of anammox consortia under long-term 1,4-dioxane exposure and recovery potential: N-kinetics and microbial dynamics
Fatigue of anammox consortia under long-term 1,4-dioxane exposure and recovery potential: N-kinetics and microbial dynamics
复制标题
长期 1,4-二恶烷暴露下厌氧氨氧化菌群的疲劳和恢复潜力：N 动力学和微生物动力学
DOI：
10.1016/j.jhazmat.2021.125533
复制
发表时间：
2021-03-10
影响因子：
13.6
通讯作者：
Elsamadony, Mohamed
中科院分区：
文献类型：
--
作者：
Ismail, Sherif;Elreedy, Ahmed;Elsamadony, Mohamed
采用周期厌氧折流板反应器(PABR)研究了不同浓度的1，4-二恶烷对厌氧氨氧化过程的长期曝露。结果表明，PABR(由4个隔室组成)对10 mg-二恶烷/L具有较强的抗性，第一个隔室通过分泌高达152.9 mg/gVSS的胞外聚合物来保护后续隔室免受1，4-二恶烷的毒害，但当1，4-二恶烷浓度增加到50 mg/L时，厌氧氨氧化细菌受到显著抑制，例如，在14天内，几乎93%的总脱氮损失。该剂量对厌氧氨氧化过程的抑制很可能是由于细菌的细胞裂解，导致第一隔室的胞外多糖分泌和比厌氧氨氧化活性分别下降到105.9 mg/gVSS和0.04mgN/gVSS/h。然而，厌氧氨氧化细菌在停止接触1，4-二恶烷后41天内成功自我恢复。微生物组成的鉴定进一步强调了1，4-二恶烷对样品中溴隐杆线虫丰度的负面影响。此外，在第一个隔室中不断观察到1，4-二恶烷被清除的扁球菌属的发展，突出了它作为缺氧1，4-二恶烷降解者的潜在作用。总体而言，长期接触1，4-二恶烷应控制在不超过10毫克/L才能成功应用。
Long-term exposure of anammox process to 1,4-dioxane was investigated using periodic anammox baffled reactor (PABR) under different 1,4-dioxane concentrations. The results generally indicated that PABR (composed of 4 compartments) has robust resistance to 10 mg-dioxane/L. The 1st compartment acted as a shield to protect subsequent compartments from 1,4-dioxane toxicity through secretion of high extracellular polymeric substance (EPS) of 152.9 mg/gVSS at 10 mg-dioxane/L. However, increasing 1,4-dioxane to 50 mg/L significantly inhibited anammox bacteria; e.g., similar to 93% of total nitrogen removal was lost within 14 days. The inhibition of anammox process at this dosage was most likely due to bacterial cell lysis, resulting in the decrease of EPS secretion and specific anammox activity (SAA) to 105.9 mg/gVSS and 0.04 mg N/gVSS/h, respectively, in the 1st compartment. However, anammox bacteria were successfully self-recovered within 41 days after the cease of 1,4-dioxane exposure. The identification of microbial compositions further emphasized the negative impacts of 1,4-dioxane on abundance of C. Brocadia among samples. Furthermore, the development of genus Planococcus in the 1st compartment, where removal of 1,4-dioxane was consistently observed, highlights its potential role as anoxic 1,4-dioxane degrader. Overall, long-term exposure to 1,4-dioxane should be controlled not exceeding 10 mg/L for a successful application.