Effects of NO3 deficiency and NaCl stress on suberin deposition in rhizo- and hypodermal (RHCW) and endodermal cell walls (ECW) of castor bean (Ricinus communis L.) roots
Effects of NO3 deficiency and NaCl stress on suberin deposition in rhizo- and hypodermal (RHCW) and endodermal cell walls (ECW) of castor bean (Ricinus communis L.) roots
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DOI：
10.1007/s11104-004-0721-6
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发表时间：
2005-02
期刊：
影响因子：
4.9
通讯作者：
L. Schreiber;R. Franke;Klaus Hartmann
中科院分区：
文献类型：
--
作者：
L. Schreiber;R. Franke;Klaus Hartmann
蓖麻（Ricinus communisL.）水培植物以达到NO3缺乏（N饥饿）、盐胁迫（加入100 mM NaCl）或正常条件。从根中酶解分离出内皮层（ECW）和根皮层和下皮层细胞壁（RHCW），并在溶剂提取后通过酯交换释放木栓素单体。芳香族和脂肪族木栓素单体进行了鉴定和定量的气相色谱和质谱。90 - 95%的释放的木栓素单体是平均链长为19个碳原子的线性长链脂肪族化合物（醇、酸、二酸、ω-羟基酸和2-羟基酸）。剩余部分是主要由香豆酸和阿魏酸组成的芳香族木栓质馏分。在NaCl的存在下，在ECW和RHCW中的苏合香素量显着增加。与此相反，N饥饿导致显着降低水平的木栓化ECW和RHCW。结论是R. communisplants加强其质外体运输障碍根在适应NaCl胁迫，以减少NaCl的吸收。在氮饥饿条件下，情况正好相反，植物减少了质外体运输障碍的木栓化，以促进从土壤中吸收养分。
Castor bean (Ricinus communisL.) plants were hydroponically cultivated to achieve NO3deficiency (N starvation), salt stress (addition of 100 mM NaCl), or normal conditions. Endodermal (ECW) and rhizodermal and hypodermal cell walls (RHCW) were isolated enzymatically from roots, and suberin monomers were released by transesterification after solvent extraction. Aromatic and aliphatic suberin monomers were identified and quantified by gas chromatography and mass spectrometry. Between 90 and 95% of the released suberin monomers were linear, long-chain, aliphatic compounds (alcohols, acids, diacids, ω-hydroxy acids and 2-hydroxy acids) with an average chain length of 19 C-atoms. The remainder was an aromatic suberin fraction mainly composed of coumaric and ferulic acid. Suberin amounts were significantly increased in ECW and RHCW in the presence of NaCl. In contrast, N starvation led to significantly reduced levels of suberization in ECW and RHCW. It is concluded thatR. communisplants reinforce their apoplastic transport barriers in roots in adaptation to NaCl stress in order to minimize NaCl uptake. Under conditions of N starvation the opposite occurs and plants reduce the suberization of their apoplastic transport barriers to facilitate nutrient uptake form the soil.