Axotomy induces a different modulation of both low‐affinity nerve growth factor receptor and choline acetyltransferase between adult rat spinal and brainstem motoneurons
Axotomy induces a different modulation of both low‐affinity nerve growth factor receptor and choline acetyltransferase between adult rat spinal and brainstem motoneurons
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轴切术诱导成年大鼠脊髓和脑干运动神经元之间低亲和力神经生长因子受体和胆碱乙酰转移酶的不同调节
DOI：
10.1002/cne.903630207
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发表时间：
1995
影响因子：
2.5
通讯作者：
P. Tonali
中科院分区：
文献类型：
--
作者：
M. Rende;I. Giambanco;Marina Buratta;P. Tonali
成年大鼠脊髓和脑干运动神经元在轴突切断后重新表达低亲和力神经生长因子受体（p75）。我们先前已经报道并量化了几种类型的坐骨神经损伤后脊髓运动神经元中这种再表达的时间过程。其他研究报道了p75在轴突切断的脑干运动神经元中的再表达。这些先前的研究结果不同的类型最有效的触发损伤p75再表达，这种再表达的相对持续时间和胆碱乙酰转移酶（ChAT）免疫反应性（-IR）的减少后，脊髓或脑干运动神经元的永久性轴突切断。这些差异表明，这两个群体的运动神经元响应轴突切断与不同的调制p75和ChAT的表达。本研究的目的是确定是否存在差分调制。我们已经分析和量化了在相同类型的损伤和我们先前用于坐骨神经运动神经元的相同时间过程后，舌下神经核中p75-和ChAT-IR运动神经元的存在。结果表明，神经挤压是最有效的触发损伤p75，它诱导类似的时间模式的p75和ChAT表达坐骨神经和舌下神经运动神经元。相反，坐骨神经和舌下神经的切割损伤导致这两个群体的运动神经元之间的p75和ChAT表达的不同的时间过程。事实上，舌下神经运动神经元的永久性轴突切断诱导了（i）p75的维持期比坐骨神经运动神经元长得多，（ii）ChAT-IR逐渐丧失，随后恢复到正常值，而坐骨神经运动神经元则适度减少。这一证据表明，脊髓和脑干运动神经元响应于永久性轴突切断与不同的调制p75和ChAT的表达。总而言之，目前的数据和报告的差异轴突切断后细胞死亡的证据支持这两个群体的运动神经元在轴突切断后经历不同的动态变化的假设。© 1995 Wiley利斯公司
Adult rat spinal and brainstem motoneurons reexpress low‐affinity nerve growth factor receptor (p75) after their axotomy. We have previously reported and quantified the time course of this reexpression in spinal motoneurons following several types of injuries of the sciatic nerve. Other studies reported the reexpression of p75 in axotomized brainstem motoneurons. Results of these previous studies differed regarding the type of the most effective triggering injury for p75 reexpression, the relative duration of this reexpression and the decrease of choline acetyltransferase (ChAT) immunoreactivity (‐IR) following a permanent axotomy of spinal or brainatem motoneurons. These differences suggest that these two populations of motoneurons respond to axotomy with a different modulation of p75 and ChAT expression. The aim of the present study was to determine whether differential modulation exists. We have analyzed and quantified the presence of p75‐ and ChAT‐IR motoneurone in the hypoglossal nucleus following the same types of injury and the same time course we previously used for sciatic motoneurons. The results show that a nerve crush is the most effective triggering injury for p75 and that it induces similar temporal patterns of p75 and ChAT expression for sciatic and hypoglossal motoneurons. In contrast, a cut injury of the sciatic and hypoglossal nerves resulted in distinct temporal courses of both p75 and ChAT expression between these two populations of motoneurons. In fact, a permanent axotomy of the hypoglossal motoneurons induced (i) a much longer maintenance phase for p75 than in aciatic motoneurons and (ii) a progressive loss of ChAT‐IR with a successive return to normal values in contrast to the modest decrease in the sciatic motoneurons. This evidence indicates that spinal and brainstem motoneurons respond to a permanent axotomy with a different modulation of p75 and ChAT expression. Altogether, the present data and the reported evidence of a differential post‐ axotomy cell death support the hypothesis that these two populations of motoneurons undergo different dynamic changes after axotomy. © 1995 Wiley‐Liss, Inc.
神经营养素对体内哺乳动物运动神经元的影响。
DOI：
10.1002/neu.480241202
发表时间：
1993
期刊：
Journal of neurobiology
影响因子：
--
作者：
Yan,Q;Elliott,JL;Matheson,C;Sun,J;Zhang,L;Mu,X;Rex,KL;Snider,WD
通讯作者：
Snider,WD