Differences in Gastric Mucosal Microbiota Profiling in Patients with Chronic Gastritis, Intestinal Metaplasia, and Gastric Cancer Using Pyrosequencing Methods
Differences in Gastric Mucosal Microbiota Profiling in Patients with Chronic Gastritis, Intestinal Metaplasia, and Gastric Cancer Using Pyrosequencing Methods
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DOI：
10.1111/hel.12145
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发表时间：
2014-12-01
期刊：
影响因子：
4.4
通讯作者：
Kim, Jihyun F.
中科院分区：
文献类型：
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作者：
Eun, Chang Soo;Kim, Byung Kwon;Kim, Jihyun F.
背景幽门螺杆菌（Helicobacter pylori，H.pylori）感染在癌症的早期发展中起重要作用.然而，促进活性氧和氮物质合成的各种细菌可能参与后期阶段。我们的目的是确定从慢性胃炎，肠上皮化生和胃癌患者的胃粘膜的微生物组成，使用454 GS FLX Titanium.MethodsGastric粘膜活检样品收集了31例患者在内镜检查。提取基因组DNA后，扩增16S rRNA基因可变区V5。采用454高通量测序仪对PCR产物进行测序。的组成，多样性，和丰富的微生物群落进行了比较，三groups.ResultsThe组成的幽门螺杆菌含有Epsilonproteobacteria类似乎是最普遍的，但相对增加的杆菌类在胃癌组注意到，导致与慢性胃炎组相比，有显着差异。从家系水平分析，胃癌组中螺旋杆菌科的相对丰度明显低于慢性胃炎组和肠化生组，而链球菌科的相对丰度明显高于慢性胃炎组和肠化生组。在基于UniFrac距离的幽门螺杆菌优势组UPGMA聚类中，慢性胃炎组和胃癌组明显分离，而肠上皮化生组分布在两组之间。结论在幽门螺杆菌占优势的患者中，胃癌患者胃粘膜的微生物组成与慢性胃炎和肠上皮化生患者有显著差异。胃微生物组成的这些改变可能在幽门螺杆菌占优势的患者的胃癌发生中发挥重要的，尚未确定的作用。
BackgroundHelicobacter pylori (H.pylori) infection plays an important role in the early stage of cancer development. However, various bacteria that promote the synthesis of reactive oxygen and nitrogen species may be involved in the later stages. We aimed to determine the microbial composition of gastric mucosa from the patients with chronic gastritis, intestinal metaplasia, and gastric cancer using 454 GS FLX Titanium.MethodsGastric mucosal biopsy samples were collected from 31 patients during endoscopy. After the extraction of genomic DNA, variable region V5 of the 16S rRNA gene was amplified. PCR products were sequenced using 454 high-throughput sequencer. The composition, diversity, and richness of microbial communities were compared between three groups.ResultsThe composition of H.pylori-containing Epsilonproteobacteria class appeared to be the most prevalent, but the relative increase in the Bacilli class in the gastric cancer group was noticed, resulting in a significant difference compared with the chronic gastritis group. By analyzing the Helicobacter-dominant group at a family level, the relative abundance of Helicobacteraceae family was significantly lower in the gastric cancer group compared with chronic gastritis and intestinal metaplasia groups, while the relative abundance of Streptococcaceae family significantly increased. In a UPGMA clustering of Helicobacter-dominant group based on UniFrac distance, the chronic gastritis group and gastric cancer group were clearly separated, while the intestinal metaplasia group was distributed in between the two groups. The evenness and diversity of gastric microbiota in the gastric cancer group was increased compared with other groups.ConclusionsIn Helicobacter predominant patients, the microbial compositions of gastric mucosa from gastric cancer patients are significantly different to chronic gastritis and intestinal metaplasia patients. These alterations of gastric microbial composition may play an important, as-yet-undetermined role in gastric carcinogenesis of Helicobacter predominant patients.