GENETIC-MAPPING OF 2 NEW BLOOD-PRESSURE QUANTITATIVE TRAIT LOCI IN THE RAT BY GENOTYPING ENDOTHELIN SYSTEM GENES
GENETIC-MAPPING OF 2 NEW BLOOD-PRESSURE QUANTITATIVE TRAIT LOCI IN THE RAT BY GENOTYPING ENDOTHELIN SYSTEM GENES
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DOI：
10.1172/jci117284
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发表时间：
1994-06-01
影响因子：
15.9
通讯作者：
RAPP, JP
中科院分区：
文献类型：
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作者：
DENG, AY;DENE, H;RAPP, JP
内皮素系统由一系列有效的血管收缩肽及其受体组成，在控制血压方面具有潜在的重要作用。我们发现，内皮素-2(ET2)，也被称为血管活性肠肽的基因编码，在来自达尔盐敏感(S)大鼠和刘易斯（LEW/NCrlBR） （LEW）大鼠杂交的F-2群体[F-2(S x LEW)]中与收缩压强烈共分离。ET2位点定位在大鼠第5染色体上。在F-2（S x LEW）群体中，睾丸特异性组蛋白（HITH）位点也与血压密切共分离，并被分配到大鼠第17染色体上。构建了5号和17号染色体上含有血压数量性状位点（QTL）区域的遗传图谱，并利用MAPMAKER/QTL程序对QTL进行定位。在几个测试的F-2种群中，内皮素-1、内皮素-3和内皮素受体A的大鼠基因与血压没有共分离，它们分别被分配到大鼠染色体17、3和19上。内皮素受体B与血压共分离弱，并暂时分配到大鼠第15染色体。我们得出结论，在大鼠中，一个新的血压QTL位于ET2位点标记的5号染色体上，另一个新的QTL位于靠近HITH位点的17号染色体上。
The endothelin system, consisting of a series of potent vasoconstrictor peptides and their receptors, is potentially important in the control of blood pressure. We found that the gene coding for endothelin-2 (ET2), also known as vasoactive intestine peptide, cosegregated strongly with systolic blood pressure in a F-2 population [F-2(S x LEW)] derived from a cross of the Dahl salt-sensitive (S) rat and the Lewis (LEW/NCrlBR) (LEW) rat. The ET2 locus was assigned to rat chromosome 5. The testis-specific histone (HITH) locus also strongly cosegregated with blood pressure in the F-2(S x LEW) population and was assigned to rat chromosome 17. Genetic maps of the regions containing the quantitative trait loci (QTL) for blood pressure on chromosomes 5 and 17 were constructed and the QTL were localized using the MAPMAKER/QTL program. The rat genes for endothelin-1, endothelin-3, and endothelin receptor A did not cosegregate with blood pressure in several F-2 populations tested and were assigned to rat chromosomes 17, 3, and 19, respectively. Endothelin receptor B cosegregated weakly with blood pressure and was provisionally assigned to rat chromosome 15. We conclude that, in the rat, one new blood pressure QTL is located on chromosome 5 marked by the ET2 locus and another new QTL is located on chromosome 17 near the HITH locus.