Use of antibodies to modify the effect of growth hormone on sugar transport in rat diaphragm muscle.
Use of antibodies to modify the effect of growth hormone on sugar transport in rat diaphragm muscle.
复制标题
使用抗体改变生长激素对大鼠膈肌糖转运的影响。
DOI：
10.1210/endo-105-2-452
复制
发表时间：
1979
期刊：
影响因子：
4.8
通讯作者：
C. Reagan
中科院分区：
文献类型：
--
作者：
O. Isaksson;J. Schwartz;J. Kostyo;C. Reagan
GH刺激糖转运到垂体切除大鼠的膈肌中，但这种作用在2-3小时内消退，随后出现一段不应期，其中细胞对激素的进一步刺激没有反应。为了确定诱导1）GH对3-O-甲基葡萄糖（30 MG）转运的早期刺激作用和2）延迟的不应性反应所需的体外GH-组织相互作用的最长时间，在不同时间用抗GH抗体中断GH-组织相互作用。将从切除垂体的大鼠分离的膈肌与大鼠GH（rGH）（0.4 μg/ml）在0-2 ℃下孵育30分钟，以允许激素在没有代谢的情况下与细胞结合。接着，在rGH存在下，将肌肉加热至37 ℃，保持不同时间，然后再次冷却至2 ℃，并暴露于猴血清或猴抗-rGH抗体（ArGH）中1小时.然后将肌肉在缓冲液中洗涤并再次加热至37 ℃不同时间.他们运送30 MG的能力是屁股...
GH stimulates sugar transport into diaphragm muscle of hypophysectomized rats, but this effect subsides in 2–3 h and a period of refractoriness follows in which the cells do not respond to further stimulation with the hormone. To determine the briefest period of in vitro GH-tissue interaction required to induce 1) the early stimulatory effect of GH on the transport of 3-O-methylglucose (30MG) and 2) the delayed refractory response, the GH-tissue interaction was interrupted at various times with antibodies against GH. Diaphragms isolated from hypophysectomized rats were incubated with rat GH (rGH), 0.4 μg/ml for 30 min at 0-2 C, to permit binding of the hormone to the cells in the absence of metabolism. Next, the muscles were warmed to 37 C for various times in the presence of rGH, then cooled again to 2 C and exposed for 1 h to either monkey serum or monkey anti-rGH antibodies (ArGH). The muscles were then washed in buffer and again warmed to 37 C for various times. Their ability to transport 30MG was ass...