pH-Responsive Fe3O4 Nanopartilces-Capped Mesoporous Silica Supports for Protein Delivery
pH-Responsive Fe3O4 Nanopartilces-Capped Mesoporous Silica Supports for Protein Delivery
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pH 响应型 Fe3O4 纳米颗粒封端介孔二氧化硅支持蛋白质输送
DOI：
10.1166/jnn.2016.11744
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发表时间：
2016
影响因子：
--
通讯作者：
Liu Changsheng
中科院分区：
文献类型：
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作者：
Gan Qi;Zhu Jiaoyang;Yuan Yuan;Liu Changsheng
目前，递送具有良好生物活性和缓释的蛋白质和多肽仍然是一个巨大的挑战。在本研究中，合成并研究了将直径为0.6-1 μm、孔径为6.2 nm的8纳米Fe3O4纳米颗粒（NPs）锚定在SBA-15载体上的ph响应递送系统。pH刺激反应是基于锚定在介孔二氧化硅支架孔出口的三（氨基甲基）乙烷（TAE）基团与包被在Fe3O4 NPs上的羧基苯甲醛（CBA）基团之间的相互作用，在酸性条件下（pH = 5）快速释放，随着pH值的增加（pH = 7.4）零释放。以BMP-2为模型蛋白，Fe3O4纳米颗粒覆盖的介孔二氧化硅对pH的变化有快速的响应。此外，释放的BMP-2仍能保持其生物活性，诱导骨髓间充质干细胞成骨分化。此外，磁性取向主要归因于Fe3O4 NPs作为纳米帽。骨髓间充质干细胞模型细胞的MTT实验证明其具有良好的生物相容性。这些结果表明，Fe3O4 nps覆盖的SBA-15材料对BMP-2等大分子蛋白具有有效的负载作用，在ph响应控释系统中具有良好的应用前景。
Delivery of proteins and peptides with excellent bioactivity and controlled release still is a great challenge nowadays. In this study, a pH-responsive delivery system obtained by anchoring 8-nm Fe3O4 nanoparticles (NPs) onto SBA-15 supports with a particle diameter in the range of 0.6–1 μm and a pore size of 6.2 nm was synthesized and investigated. The pH-stimulative response is based on the interaction between the tris(aminomethyl)ethane (TAE) groups anchored onto the pore outlet of mesoporous silica scaffolds and the carboxybenzaldehyde (CBA) groups coated on the Fe3O4 NPs, which can lead to a rapid release under the acid condition (pH = 5) and a zero release with the increase of pH value (pH = 7.4). With BMP-2 as a model protein, this Fe3O4 nanopartilces-capped mesoporous silica showed a rapid response to the change of pH for protein delivery. Furthermore, the released BMP-2 could still maintain its bioactivity and induce the osteoblast differentiation of BMSCs. Besides, the magnetic orientation mainly attributes to the Fe3O4 NPs served as the nanocaps. The excellent bio-compatibility is demonstrated by the MTT assay on BMSCs model cells. These results show that Fe3O4 NPs-capped SBA-15 materials have an effective load for large molecule size proteins, such as BMP-2, and show an excellent applied prospect in pH-responsive controlled release system.