Genome-wide survey of Aux/IAA gene family members in potato (Solanum tuberosum): Identification, expression analysis, and evaluation of their roles in tuber development
Genome-wide survey of Aux/IAA gene family members in potato (Solanum tuberosum): Identification, expression analysis, and evaluation of their roles in tuber development
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马铃薯（Solanum tuberosum）Aux/IAA 基因家族成员的全基因组调查：鉴定、表达分析和评估其在块茎发育中的作用
DOI：
10.1016/j.bbrc.2016.02.013
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发表时间：
2016-03-04
影响因子：
3.1
通讯作者：
Ma, Haoli
中科院分区：
文献类型：
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作者：
Gao, Junpeng;Cao, Xiaoli;Ma, Haoli
生长素/吲哚-3-乙酸（Aux/IAA）基因编码已知参与对生长素的初级细胞反应的短寿命核蛋白。迄今为止，尚未对马铃薯（Solanum tuberosum）中的Aux/IAA基因进行系统分析。本研究共鉴定了26个马铃薯Aux/IAA基因（命名为StIAA 1 ~ StIAA 26），并基于多重序列比对和基于模体的序列分析，分析了StIAAs共有的4个保守结构域的分布。对马铃薯和拟南芥的Aux/IAA基因家族进行了系统发育分析。为了研究StIAA基因在马铃薯块茎发育中的作用，将RNA-seq研究结果重新格式化，分析StIAA基因的表达模式，并通过实时荧光定量PCR进行验证。大量的StIAA基因（12个）在匍匐茎器官和块茎起始和膨大发育阶段中高表达，其中大部分基因对吲哚乙酸处理有反应。研究结果表明，StIAA基因参与了马铃薯块茎的发育过程，并为马铃薯Aux/IAA基因的功能研究提供了新的思路。(C)2016 Elsevier Inc. All rights reserved.
The Auxin/indole-3-acetic acid (Aux/IAA) genes encode short-lived nuclear proteins that are known to be involved in the primary cellular responses to auxin. To date, systematic analysis of the Aux/IAA genes in potato (Solanum tuberosum) has not been conducted. In this study, a total of 26 potato Aux/IAA genes were identified (designated from StIAA1 to StIAA26), and the distribution of four conserved domains shared by the StIAAs were analyzed based on multiple sequence alignment and a motif-based sequence analysis. A phylogenetic analysis of the Aux/IAA gene families of potato and Arabidopsis was also conducted. In order to assess the roles of StIAA genes in tuber development, the results of RNA-seq studies were reformatted to analyze the expression patterns of StIAA genes, and then verified by quantitative real-time PCR. A large number of StIAA genes (12 genes) were highly expressed in stolon organs and in during the tuber initiation and expansion developmental stages, and most of these genes were responsive to indoleacetic acid treatment. Our results suggested that StIAA genes were involved in the process of tuber development and provided insights into functional roles of potato Aux/IAA genes. (C) 2016 Elsevier Inc. All rights reserved.