A PROGRAMMABLE SYSTEM TO PERFORM THE POLYMERASE CHAIN-REACTION
A PROGRAMMABLE SYSTEM TO PERFORM THE POLYMERASE CHAIN-REACTION
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DOI：
10.1089/dna.1.1988.7.441
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发表时间：
1988-07-01
期刊：
影响因子：
--
通讯作者：
GRAY, JW
中科院分区：
文献类型：
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作者：
WEIER, HU;GRAY, JW
描述了一种使用聚合酶链式反应(PCR)在体外进行特定DNA片段的酶扩增所需的循环温度变化的自动化系统。在聚合酶链式反应过程中，寡核苷酸引物分子在低温下结合到热变性的DNA模板上，并使用耐热的DNA聚合酶延伸到其3‘’端。在程序控制下，DNA变性、引物退火和延伸重复数次，以积累大量相同的DNA序列副本。微电脑系统控制96度的流量。C和37度。C水通过24孔样品保持器，从而使保持器中样品的温度根据DNA变性、底物退火和DNA聚合所需的不同而变化。微型计算机自动执行多个热循环，并且足够灵活，温度分布可以在不同的循环中改变。
An automated system is described that performs the cyclic temperature changes required for enzymatic amplication of specific DNA segments in vitro using the polymerase chain reaction (pcr). During pcr, oligonucleotide primer molecules are bound at low temperature to templates of heat-denatured DNA and extended on their 3'' end using a thermostable DNA polymerase. The DNA denaturation, primer annealing, and extension is repeated several times under program control to accumulate a large number of identical copies of the DNA sequence between the primers. A microcomputer system controls the flow of 96.degree. C and 37.degree. C water through a 24-well sample holder so that the temperature in the samples in the holder varies as required for DNA denaturation, primer annealing, and DNA polymerization. The microcomputer automatically performs multiple thermal cycles and is sufficiently flexible that the temperature profile can be varied from cycle to cycle.