Role of cyclic strain frequency in regulating the alignment of vascular smooth muscle cells in vitro
Role of cyclic strain frequency in regulating the alignment of vascular smooth muscle cells in vitro
复制标题
循环应变频率在体外调节血管平滑肌细胞排列中的作用。
DOI：
10.1529/biophysj.106.098574
复制
发表时间：
2008-02-15
影响因子：
3.4
通讯作者：
Jiang, Zong-Lai
中科院分区：
文献类型：
--
作者：
Liu, Bo;Qu, Ming-Juan;Jiang, Zong-Lai
由于血压的周期性变化，动脉系统受到周期性应变，但周期性应变频率对动脉平滑肌细胞(SMCs)的影响尚不清楚。在这里，我们使用体外模型研究了循环应变频率在调节SMC排列中的潜在作用。使用Flexcell TRANSION Plus系统对主动脉SMC施加一个伸长的循环应变，但以不同的频率施加。研究发现，微丝的角度信息熵、整合素-β1活性、p38MAPK活性和F/G肌动蛋白比值均随频率发生变化，这与不同频率循环应变下SMC的排列规律一致。用抗整合素-β1抗体、SB202190或细胞松弛素D处理可抑制依赖于应变频率的循环SMC排列。这些观察结果表明，循环应变的频率在调节完整的肌动蛋白细丝依赖的血管SMC排列中起作用，1.25赫兹的循环应变是影响SMC排列的最有效频率。此外，整合素-β1和p38MAPK可能介导了周期性应变频率依赖性的SMC对齐。
The arterial system is subjected to cyclic strain because of periodic alterations in blood pressure, but the effects of frequency of cyclic strain on arterial smooth muscle cells (SMCs) remain unclear. Here, we investigated the potential role of the cyclic strain frequency in regulating SMC alignment using an in vitro model. Aortic SMCs were subject to cyclic strain at one elongation but at various frequencies using a Flexercell Tension Plus system. It was found that the angle information entropy, the activation of integrin-beta1, p38 MAPK, and F/G actin ratio of filaments were all changed in a frequency-dependent manner, which was consistent with SMC alignment under cyclic strain with various frequencies. A treatment with anti-integrin-beta1 antibody, SB202190, or cytochalasin D inhibited the cyclic strain frequency-dependent SMC alignment. These observations suggested that the frequency of cyclic strain plays a role in regulating the alignment of vascular SMCs in an intact actin filament-dependent manner, and cyclic strain at 1.25 Hz was the most effective frequency influencing SMC alignment. Furthermore, integrin-beta1 and p38 MAPK possibly mediated cyclic strain frequency-dependent SMC alignment.