NEURAL CELL-ADHESION MOLECULE IS ON EMBRYONIC MUSCLE-CELLS AND MEDIATES ADHESION TO NERVE-CELLS INVITRO
NEURAL CELL-ADHESION MOLECULE IS ON EMBRYONIC MUSCLE-CELLS AND MEDIATES ADHESION TO NERVE-CELLS INVITRO
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DOI：
10.1038/295693a0
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发表时间：
1982-01-01
期刊：
影响因子：
64.8
通讯作者：
EDELMAN, GM
中科院分区：
文献类型：
--
作者：
GRUMET, M;RUTISHAUSER, U;EDELMAN, GM
神经与肌肉的相互作用，特别是在神经肌肉接头处，是神经生理学研究最广泛的领域之一。然而，从发育生物学的角度来看，人们对导致这些连接形成的细胞和分子事件知之甚少1，2。在之前对鸡胚细胞间粘附的研究中，我们在几乎所有神经细胞的表面上发现了一种分子，称为神经细胞粘附分子（N-CAM）3，4，它似乎是细胞间结合形成的配体5。N-CAM参与多种发育过程，包括神经突成束和视网膜组织中丛状和细胞层的形成6 -8。我们在这里报告，N-CAM抗原决定簇也存在于鸡胚骨骼肌细胞。这一观察结果提出了N-CAM参与神经和肌肉细胞之间粘附的可能性。在为探索这种可能性而建立的模型系统中，我们观察到成肌细胞与视网膜神经细胞的体外粘附，并且这种粘附可被抗N-CAM抗体的F（ab′）片段抑制。此外，从鸡胚脑获得的膜囊泡或由脂质和亲和纯化的N-CAM 9重构的人工囊泡5与成肌细胞和肌管表面结合，这种结合也被抗N-CAM F（ab′）阻断。这些结果表明，N-CAM粘附系统在体外可促进神经-肌肉以及神经-神经相互作用。
The interaction of nerves with muscles, particularly at neuromuscular junctions, is one of the most extensively studied areas of neurophysiology. From the point of view of developmental biology, however, little is known about the cellular and molecular events that lead to formation of these junctions1,2. In previous investigations of cell-cell adhesion in the chick embryo, we identified a molecule on the surface of essentially all nerve cells, called neural cell adhesion molecule (N-CAM)3,4, that appears to be a ligand in the formation of cell-cell bonds5. N-CAM is involved in a variety of developmental processes including neurite fasciculation and the formation of plexiform and cell layers in retinal tissue6–8. We report here that N-CAM antigenic determinants are also present on chick embryo skeletal muscle cells. This observation raised the possibility that N-CAM is involved in adhesion between nerve and muscle cells. In model systems set up to explore this possibility, we observed that myoblasts will adherein vitroto nerve cells from retina, and that this adhesion can be inhibited by F(ab′) fragments of antibodies against N-CAM. In addition, membrane vesicles obtained from chick embryo brain or artificial vesicles5reconstituted from lipid and affinity-purified N-CAM9bound to the surface of myoblasts and myotubes; this binding was also blocked by anti-N-CAM F(ab′). These results suggest that the N-CAM adhesion system can facilitate nerve-muscle as well as nerve-nerve interactionsin vitro.