Vibrationally resonant sum-frequency generation microscopy with a solid immersion lens.
Vibrationally resonant sum-frequency generation microscopy with a solid immersion lens.
复制标题
DOI：
10.1364/boe.5.002125
复制
发表时间：
2014-07
影响因子：
3.4
通讯作者：
Eun Seong Lee;Sang-Won Lee;Julie Hsu;E. Potma
中科院分区：
文献类型：
--
作者：
Eun Seong Lee;Sang-Won Lee;Julie Hsu;E. Potma
我们使用半球形蓝宝石透镜结合离轴抛物面镜来演示中红外范围内的高分辨率振动共振和频率生成（VR-SFG）显微镜。当蓝宝石透镜作为浸没固体介质时，抛物面镜物镜的数值孔径（NA）提高了1.72倍，从0.42提高到0.72，接近理论值1.76 （= nsapphire）。测量的横向分辨率高达0.64 μm。我们通过使用和不使用固体浸没透镜成像富含胶原蛋白的组织来展示蓝宝石浸没透镜的实际用途。
We use a hemispheric sapphire lens in combination with an off-axis parabolic mirror to demonstrate high-resolution vibrationally resonant sum-frequency generation (VR-SFG) microscopy in the mid-infrared range. With the sapphire lens as an immersed solid medium, the numerical aperture (NA) of the parabolic mirror objective is enhanced by a factor of 1.72, from 0.42 to 0.72, close to the theoretical value of 1.76 ( = nsapphire). The measured lateral resolution is as high as 0.64 μm. We show the practical utility of the sapphire immersion lens by imaging collagen-rich tissues with and without the solid immersion lens.