Encoding of whisker input by cerebellar Purkinje cells
Encoding of whisker input by cerebellar Purkinje cells
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DOI：
10.1113/jphysiol.2010.195180
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发表时间：
2010-10-01
影响因子：
5.5
通讯作者：
De Zeeuw, Chris I.
中科院分区：
文献类型：
--
作者：
Bosman, Laurens W. J.;Koekkoek, Sebastiaan K. E.;De Zeeuw, Chris I.
小脑皮质对于感觉运动整合至关重要。感觉运动输入通过两条传入通路会聚在小脑浦肯野细胞上：触发复杂尖峰的攀爬纤维通路和调节浦肯野细胞简单尖峰活动的苔藓纤维平行纤维通路。我们使用，为第一次，小鼠胡须系统作为一个模型系统来研究浦肯野细胞的体感输入的编码。我们发现，大多数浦肯野细胞在同侧海马1区和海马2区的清醒小鼠响应晶须刺激与复杂的尖峰和/或简单的尖峰反应。麻醉小鼠的单须刺激揭示了复杂锋电位和简单锋电位反应的感受野是惊人的不同。复合锋电位反应，这被证明是敏感的幅度，速度和方向的胡须运动，引起只有一个或几个胡须。许多个体的胡须都能诱发不受运动方向影响的简单锋电位反应。浦肯野细胞的感受野在很大程度上是混合的，我们认为这有利于快速整合来自不同来源的感觉输入。此外，我们描述了单个浦肯野细胞，至少在麻醉下，可以绑定在两个功能合奏的基础上的感受野和同步的复杂的尖峰和简单的尖峰响应。“复杂的穗合奏”定向在矢状面，以下的解剖组织的攀爬纤维，而“简单的穗合奏”定向在横向平面，是平行的纤维束。
The cerebellar cortex is crucial for sensorimotor integration. Sensorimotor inputs converge on cerebellar Purkinje cells via two afferent pathways: the climbing fibre pathway triggering complex spikes, and the mossy fibre-parallel fibre pathway, modulating the simple spike activities of Purkinje cells. We used, for the first time, the mouse whisker system as a model system to study the encoding of somatosensory input by Purkinje cells. We show that most Purkinje cells in ipsilateral crus 1 and crus 2 of awake mice respond to whisker stimulation with complex spike and/or simple spike responses. Single-whisker stimulation in anaesthetised mice revealed that the receptive fields of complex spike and simple spike responses were strikingly different. Complex spike responses, which proved to be sensitive to the amplitude, speed and direction of whisker movement, were evoked by only one or a few whiskers. Simple spike responses, which were not affected by the direction of movement, could be evoked by many individual whiskers. The receptive fields of Purkinje cells were largely intermingled, and we suggest that this facilitates the rapid integration of sensory inputs from different sources. Furthermore, we describe that individual Purkinje cells, at least under anaesthesia, may be bound in two functional ensembles based on the receptive fields and the synchrony of the complex spike and simple spike responses. The 'complex spike ensembles' were oriented in the sagittal plane, following the anatomical organization of the climbing fibres, while the 'simple spike ensembles' were oriented in the transversal plane, as are the beams of parallel fibres.