Comparison of polarization properties of human adult microglia and blood-derived macrophages
Comparison of polarization properties of human adult microglia and blood-derived macrophages
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DOI：
10.1002/glia.22298
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发表时间：
2012-05-01
期刊：
影响因子：
6.2
通讯作者：
Antel, Jack P.
中科院分区：
文献类型：
--
作者：
Durafourt, Bryce A.;Moore, Craig S.;Antel, Jack P.
中枢神经系统实质中的常驻髓样细胞小胶质细胞以及浸润的血源性巨噬细胞都参与中枢神经系统的炎症反应。巨噬细胞可极化为M1和M2表型，这与包括炎症相关分子产生和吞噬活性在内的功能特性相关。我们比较了源自成人中枢神经系统的小胶质细胞与源自外周血单核细胞的巨噬细胞在M1和M2极化条件下的表型和功能特性。在M1条件下，小胶质细胞和巨噬细胞上调CCR7和CD80的表达。对小胶质细胞进行M2处理可诱导CD209的表达，但不会诱导M2巨噬细胞所表达的其他标志物CD23、CD163和CD206的表达。M1极化条件诱导小胶质细胞和巨噬细胞产生IL - 12p40；在M1条件下，小胶质细胞产生的IL - 10水平高于巨噬细胞。在M2条件下，小胶质细胞±脂多糖（LPS）产生与M1条件下相当水平的IL - 10，而在M2巨噬细胞中IL - 10是由LPS诱导产生的。在所有条件下，小胶质细胞的髓鞘吞噬作用都强于巨噬细胞；对于这两种细胞类型，M2细胞的活性都更高。我们的研究结果描绘了小胶质细胞与外源性髓样细胞相比，在对中枢神经系统炎症环境产生的信号作出反应时的独特特性。（C）2012威利期刊公司
Both microglia, the resident myeloid cells of the CNS parenchyma, and infiltrating blood-derived macrophages participate in inflammatory responses in the CNS. Macrophages can be polarized into M1 and M2 phenotypes, which have been linked to functional properties including production of inflammation association molecules and phagocytic activity. We compare phenotypic and functional properties of microglia derived from the adult human CNS with macrophages derived from peripheral blood monocytes in response to M1 and M2 polarizing conditions. Under M1 conditions, microglia and macrophages upregulate expression of CCR7 and CD80. M2 treatment of microglia-induced expression of CD209 but not additional markers CD23, CD163, and CD206 expressed by M2 macrophages. M1-polarizing conditions induced production of IL-12p40 by both microglia and macrophages; microglia produced higher levels of IL-10 under M1 conditions than did macrophages. Under M2 conditions, microglia +/- LPS produced comparable levels of IL-10 under M1 conditions whereas IL-10 was induced by LPS in M2 macrophages. Myelin phagocytosis was greater in microglia than macrophages under all conditions; for both cell types, activity was higher for M2 cells. Our findings delineate distinctive properties of microglia compared with exogenous myeloid cells in response to signals derived from an inflammatory environment in the CNS. (C) 2012 Wiley Periodicals, Inc.