Parkin affects mitochondrial function and apoptosis in neuronal and myogenic cells.
Parkin affects mitochondrial function and apoptosis in neuronal and myogenic cells.
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DOI：
10.1016/j.bbrc.2006.06.201
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发表时间：
2006-09
影响因子：
3.1
通讯作者：
Y. Kuroda;T. Mitsui;M. Kunishige;Toshio Matsumoto
中科院分区：
文献类型：
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作者：
Y. Kuroda;T. Mitsui;M. Kunishige;Toshio Matsumoto
我们研究了parkin对SH-SY5Y、L6、RD和COS-1细胞线粒体功能和细胞凋亡的影响。野生型parkin抑制SH-SY5Y细胞的ROS产生，突变型parkin增强SH-SY5Y和L6细胞的ROS产生。在parkin过度表达的细胞中，线粒体中检测到的活性氧中间产物减少。Parkin可抑制SH-SY5Y和L6细胞的凋亡并增强线粒体膜电位，而对COS-1细胞无此作用。线粒体呼吸链复合体的表达和酶活性没有均匀增强，但复合体1的表达和酶活性选择性增强。结果提示，parkin具有细胞选择性功能，即parkin在神经细胞或肌源性细胞中具有抗凋亡和抗氧化功能，而在肾脏细胞中无此作用。
We investigated the effect of parkin on mitochondrial function and apoptosis in SH-SY5Y, L6, RD, and COS-1 cells. Wild-type parkin attenuated reactive oxygen species (ROS) production in SH-SY5Y cells and mutant parkin enhanced ROS production in SH-SY5Y and L6 cells. Reactive oxygen intermediates, that were detected in mitochondria, were decreased in cells with overexpression of parkin. Parkin prevented apoptosis and enhanced mitochondrial membrane potentials in SH-SY5Y and L6 cells not in COS-1 cells. Expressions and enzymatic activities of mitochondrial respiratory chain complexes were not uniformly enhanced but those of complex 1 were selectively enhanced. The present results suggest the cell-selective function of parkin, i.e., parkin possesses anti-apoptotic and anti-oxidant function in neuronal or myogenic cells but not in kidney cells.