A plant plasma membrane Ca2+ pump is required for normal pollen tube growth and fertilization
A plant plasma membrane Ca2+ pump is required for normal pollen tube growth and fertilization
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DOI：
10.1073/pnas.0401542101
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发表时间：
2004-06-22
影响因子：
11.1
通讯作者：
Harper, JF
中科院分区：
文献类型：
--
作者：
Schiott, M;Romanowsky, SM;Harper, JF
Ca 2+信号在植物的生长发育过程中起着重要的作用，包括花粉管生长和受精的关键环节。Ca 2+信号的动力学主要由流入（通过通道）和流出（通过泵和反向转运蛋白）控制。拟南芥基因组编码14个Ca 2+泵，其中10个属于自抑制Ca 2 + ATP酶（ACA）家族，预测由Ca 2 +/钙调素激活。在这里，我们表明，亚型ACA 9主要在花粉中表达，并定位于质膜。三个独立的T-DNA（转移到植物细胞的Ti（肿瘤诱导）质粒的一部分）的ACA 9基因破坏被发现导致部分雄性不育。通过使用显示质膜定位的ACA 9-黄色荧光蛋白（YFP）融合物观察互补。突变体aca 9花粉表现出降低的生长潜力和高频率的败育，导致结实率降低>80%。这些研究结果确定了质膜Ca 2+转运蛋白作为开花植物花粉发育和受精的关键调节因子。
Ca2+ signals are thought to play important roles in plant growth and development, including key aspects of pollen tube growth and fertilization. The dynamics of a Ca2+ signal are largely controlled by influx (through channels) and efflux (through pumps and antiporters). The Arabidopsis genome encodes 14 Ca2+ pumps, 10 of which belong to a family of autoinhibited Ca2+ ATPases (ACA) that are predicted to be activated by Ca2+/calmodulin. Here, we show that isoform ACA9 is expressed primarily in pollen and localized to the plasma membrane. Three independent T-DNA [portion of the Ti (tumor-inducing) plasmid that is transferred to plant cells] gene disruptions of ACA9 were found to result in partial male sterility. Complementation was observed by using a ACA9-yellow fluorescence protein (YFP) fusion that displayed plasma membrane localization. Mutant aca9 pollen displayed a reduced growth potential and a high frequency of aborted fertilization, resulting in a >80% reduction in seed set. These findings identify a plasma membrane Ca2+ transporter as a key regulator of pollen development and fertilization in flowering plants.