Co-production of biodiesel and bioethanol using psychrophilic microalga Chlamydomonas sp. KNM0029C isolated from Arctic sea ice
Co-production of biodiesel and bioethanol using psychrophilic microalga Chlamydomonas sp. KNM0029C isolated from Arctic sea ice
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DOI：
10.1186/s13068-020-1660-z
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发表时间：
2020-02-01
影响因子：
6.3
通讯作者：
Han, Se Jong
中科院分区：
文献类型：
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作者：
Kim, Eun Jae;Kim, Sanghee;Han, Se Jong
背景利用微藻作为可持续能源生产的生物燃料受到了广泛的关注。微藻可以用二氧化碳和太阳能以低成本培养，而不会与可食用作物竞争。嗜冷微藻可以作为一种合适的原料来生产生物燃料，而不受低温环境的限制，因为它们可以在10摄氏度以下生长。然而，缺乏利用嗜冷微藻来生产生物柴油和生物乙醇的有效策略。因此，本研究旨在优化北极衣藻生产生物柴油和生物乙醇的工艺条件。KNM0029℃低温下。结果在20-L光生物反应器中培养后，用改进的FAME提取方法提取脂肪酸甲酯，最高得率为0.16g/gKNM0029C。将剩余生物质用于生物乙醇生产，并对不同方法的产率进行了比较。用酶(淀粉糖苷酶)进行超声处理，可获得最高的生物乙醇产量(0.22-g/g剩余生物量)。获得了大约300毫克的生物燃料，包括每克干细胞156毫克的FAME生物柴油和144毫克的生物乙醇，这是从嗜冷微藻中记录到的最高产量。结论这是首次尝试利用北极微藻衣藻的生物量。KNM0029C用于生物乙醇和生物柴油的联产，在已报道的使用嗜冷生物的研究中产生了最高的价值。这些结果可以作为利用极地微藻高效生产生物燃料的来源。
Background Biofuels, generated using microalgae as sustainable energy, have received a lot of attention. Microalgae can be cultivated at low cost with CO2 and solar energy without competition from edible crops. Psychrophilic microalgae can be a suitable feedstock to produce biofuels without the environmental constraints of low temperatures, because they can grow below 10 degrees C. However, there is a lack of efficient strategies using psychrophilic microalgae to produce biodiesel and bioethanol. Therefore, the current study aimed to optimize the production of biodiesel and bioethanol from Arctic Chlamydomonas sp. KNM0029C at low temperatures. Results After incubation in a 20-L photobioreactor, fatty acid methyl ester (FAME) was extracted using modified FAME extraction methods, producing a maximum yield of 0.16-g FAME/g KNM0029C. Residual biomass was pretreated for bioethanol production, and the yields from different methods were compared. The highest bioethanol yield (0.22-g/g residual biomass) was obtained by pretreatment with enzyme (amyloglucosidase) after sonication. Approximately 300-mg biofuel was obtained, including 156-mg FAME biodiesel and 144-mg bioethanol per g dried cells, representing the highest recorded yield from psychrophilic microalgae. Conclusions This is the first to attempt at utilizing biomass from psychrophilic Arctic microalga Chlamydomonas sp. KNM0029C for the co-production of bioethanol and biodiesel, and it yielded the highest values among reported studies using psychrophilic organisms. These results can be used as a source for the efficient biofuel production using polar microalgae.