MEMBRANE-ASSOCIATED PHOSPHOLIPASE-C OF DROSOPHILA RETINA
MEMBRANE-ASSOCIATED PHOSPHOLIPASE-C OF DROSOPHILA RETINA
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DOI：
10.1093/oxfordjournals.jbchem.a122246
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发表时间：
1988-01-01
影响因子：
2.7
通讯作者：
HOTTA, Y
中科院分区：
文献类型：
--
作者：
INOUE, H;YOSHIOKA, T;HOTTA, Y
用果蝇视觉突变体的头部匀浆检测了磷脂酶C对磷脂酰肌醇4，5-二磷酸和磷脂酰肌醇的活性。在许多突变体中，发现酶活性降低。对磷脂酰肌醇4，5-二磷酸和磷脂酰肌醇的活性总是受到平行的突变体之间的影响，而与磷脂酰肌醇代谢相关的其他酶，如甘油二酯激酶的活性，没有。该酶被认为是膜相关的，并在低Ca 2+浓度（10-7 M）时被最大限度地激活，当磷脂酰肌醇4，5-二磷酸被用作底物，而与磷脂酰肌醇获得的活性增加与Ca 2+浓度高达10-4 M。pH对两种酶活性的影响存在一定差异。
Phospholipase C activities against phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol have been examined using head homogenate of Drosophila visual mutants. In many mutants the enzyme activities were found to be reduced. The activities against both phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol were always affected in parallel among the mutants, while the activities of other enzyme related to phosphatidylinositol metabolism, such as diacylglycerol kinase, were not. The enzyme was concluded to be membrane-associated and was activated maximally at low Ca2+ concentration (10-7 M), when phosphatidylinositol 4,5-bisphosphate was used as a substrate, while the activity obtained with phosphatidylinositol increased with the Ca2+ concentration up to 10-4 M. The effects of pH on these two enzyme activities differed to some extent.