Determination of protoapigenone in beagle dog plasma by LC-MS/MS: Application to a pharmacokinetic study
Determination of protoapigenone in beagle dog plasma by LC-MS/MS: Application to a pharmacokinetic study
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通过 LC–MS/MS 测定比格犬血浆中的原芹菜酮：在药代动力学研究中的应用
DOI：
10.1007/s43450-021-00205-x
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Anhua Wei
中科院分区：
文献类型：
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作者：
Cheng Qin;Yongjun Zhang;Xin Jiang;Xueyan Yin;Yaping Li;Wanyu Liu;Luqin Si;Jiangeng Huang;Anhua Wei
原芹菜酮是一类独特的天然黄酮类化合物，具有显著的细胞毒和抗肿瘤活性。建立了一种简便、灵敏、快速的LC-MS/MS法测定血浆中原芹菜酮的浓度，并对该方法进行了验证，以获得原芹菜酮在比格犬体内的药代动力学曲线。采用乙酸乙酯-10%氢氧化铵（2：1，v/v）进行液-液萃取（回收率> 90%）。在最佳条件下，采用C18色谱柱，以乙腈-10 mM甲酸铵水溶液（9：1，v/v）为移动的相，流速为0.5 ml/min，在0.2 ~ 100 ng/ml范围内，该方法具有良好的线性、选择性、准确度和精密度。最后，将该方法成功应用于比格犬口服原芹菜酮后的药代动力学研究。
Protoapigenone, a unique class of natural flavonoid, exerted significant cytotoxic and antitumoral activities. A simple, sensitive, and rapid LC–MS/MS method for the quantification of protoapigenone in plasma was developed and validated to obtain the pharmacokinetic profile of protoapigenone in beagle dogs. Ethyl acetate-10% ammonium hydroxide (2:1, v/v) was employed for liquid–liquid extraction (recovery > 90%). Under optimal conditions, chromatographic resolution was achieved on a C18column using acetonitrile and 10 mM ammonium formate in water (9:1, v/v), as mobile phase at a flow rate of 0.5 ml/min. The established method presented good linearity, selectivity, accuracy, and precision in the range of 0.2–100 ng/ml. Finally, this method was successfully applied to a pharmacokinetic study in beagle dogs following oral administration of protoapigenone.Graphical abstract