Suppression of phase and amplitude J(HH) modulations in HSQC experiments
Suppression of phase and amplitude J(HH) modulations in HSQC experiments
复制标题
DOI：
10.1002/mrc.4149
复制
发表时间：
2015-02-01
影响因子：
2
通讯作者：
Parella, Teodor
中科院分区：
文献类型：
--
作者：
Castanar, Laura;Sistare, Eduard;Parella, Teodor
在传统的2D HSQC实验中，交叉峰的幅度和相位由质子-质子J（HH）和质子-碳（1）J（CH）耦合常数调制。它是由频谱模拟和实验表明，J（HH）的干扰被抑制在一个新的完美的HSQC脉冲计划，将完美回波INEPT周期。改进的二维谱提供了关于（1）J（CH）和J（HH）的纯同相交叉峰，而与实验延迟优化无关。此外，峰体积不受J（HH）的影响而衰减，从而使得诸如相位校正、多重峰分析和信号积分的实际问题更合适。版权所有（c）2014约翰威利父子有限公司
The amplitude and the phase of cross peaks in conventional 2D HSQC experiments are modulated by both proton-proton, J(HH), and proton-carbon, (1)J(CH), coupling constants. It is shown by spectral simulation and experimentally that J(HH) interferences are suppressed in a novel perfect-HSQC pulse scheme that incorporates perfect-echo INEPT periods. The improved 2D spectra afford pure in-phase cross peaks with respect to (1)J(CH) and J(HH), irrespective of the experiment delay optimization. In addition, peak volumes are not attenuated by the influence of J(HH), rendering practical issues such as phase correction, multiplet analysis, and signal integration more appropriate. Copyright (c) 2014 John Wiley & Sons, Ltd.