Early divergence and changing proportions of neuronal and glial precursor cells in the primate cerebral ventricular zone.
Early divergence and changing proportions of neuronal and glial precursor cells in the primate cerebral ventricular zone.
复制标题
灵长类脑室区神经元和神经胶质前体细胞的早期分歧和比例变化。
DOI：
10.1016/0012-1606(83)90184-7
复制
发表时间：
1983
影响因子：
2.7
通讯作者：
Rakic,P
中科院分区：
文献类型：
--
作者：
Levitt,P;Cooper,ML;Rakic,P
应用抗胶质细胞酸性蛋白（GFA）抗血清免疫细胞化学染色法，观察了恒河猴胎儿165天前半期枕叶增殖性脑室带（VZ）的细胞组成。电子显微镜分析显示，在胚胎（E）年龄E39和E40的标本中有少量的GFA阳性细胞。到E47，VZ中约28%的细胞呈免疫反应性。这一比例在E61增加到37%，到E80达到60%。GFA阳性有丝分裂像的分数遵循相同的一般趋势，分别在E47、E61和E80标记34、47和80%。本研究结果揭示了存在两个基本类的细胞，GFA阳性和GFA阴性，表明在灵长类动物的大脑，神经胶质细胞和神经元前体细胞可能共存于脑室区在胚胎年龄时，很少，如果有的话，神经元已成为有丝分裂后。
Immunocytochemical staining with antisera directed against glial fibrillary acidic protein (GFA) was used to examine the cellular composition of the proliferative ventricular zone (VZ) in the occipital lobe of rhesus monkey fetuses during the first half of their 165-day gestational period. Electron microscopic analysis revealed a small number of GFA-positive cells in specimens of embryonic (E) ages E39 and E40. By E47 about 28% of the cells in the VZ were immunoreactive. This percentage increased to 37% at E61 and reached 60% by E80. The fraction of GFA-positive mitotic figures followed the same general tendency with 34, 47, and 80% being labeled at E47, E61, and E80, respectively. The present results reveal the existence of two basic classes of cells, GFA positive and GFA negative, indicating that in the primate brain, glial and neuronal precursor cells may coexist in the ventricular zone at embryonic ages when few, if any, neurons have become postmitotic.