Induction of TNBS colitis in mice.
Induction of TNBS colitis in mice.
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DOI：
10.1002/0471142735.im1519s49
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发表时间：
2002-08-01
影响因子：
--
通讯作者：
Fuss, Ivan J
中科院分区：
文献类型：
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作者：
Scheiffele, Frank;Fuss, Ivan J
本单元描述的动物模型是用于研究炎症性肠病免疫发病机制的几种动物模型之一。该模型使用 2,4,6-三硝基苯磺酸 (TNBS)，当直肠内给予 SJL/J 小鼠时，会诱发严重的结肠炎症。由该手术引起的结肠炎的临床和组织病理学结果与克罗恩病中所见的结果相似。该单元描述了成功诱导 TNBS 结肠炎所需的关键参数以及疾病水平监测和分级的方法。还包括从小鼠结肠中分离固有层单核细胞的支持方案。
The animal model described in this unit is one of several that have been used to study the immunopathogenesis of inflammatory bowel disease. The model employs the use of 2,4,6-trinitrobenzenesulfonic acid (TNBS), which induces severe colonic inflammation when administered intrarectally in SJL/J mice. The colitis which results from this procedure presents clinical and histopathological findings that resemble those seen in Crohn's disease. The unit describes the critical parameters needed for successful induction of TNBS-colitis as well methods for monitoring and grading disease levels. A support protocol for isolating lamina propria mononuclear cells from mouse colons is also included.