Detection and identification of putative bacterial endosymbionts and endogenous viruses in tick cell lines
Detection and identification of putative bacterial endosymbionts and endogenous viruses in tick cell lines
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DOI：
10.1016/j.ttbdis.2012.05.002
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发表时间：
2012-01-01
影响因子：
3.2
通讯作者：
Bell-Sakyi, Lesley
中科院分区：
文献类型：
--
作者：
Alberdi, M. Pilar;Dalby, Matthew J.;Bell-Sakyi, Lesley
除了作为许多具有医学和兽医学重要性的病毒、细菌和原生动物病原体的载体之外，蜱虫还含有多种未知对脊椎动物宿主具有致病性的微生物。从硬蜱和硬蜱中建立的连续细胞系可以被这种内共生细菌和内源性病毒感染，但迄今为止，很少有细胞系被检测到它们的存在。从Roslin Wellcome Trust Tick Cell Biobank（http：//www.example.com）中保藏的超过50种蜱细胞系中提取的DNA和RNA分别筛选细菌和RNA病毒的存在。tickcells.roslin.ac.uk使用pan-16 S rRNA引物扩增的PCR产物的测序揭示了来自Amblyomma和Dermacentor spp的几个细胞系中细菌内共生体的DNA序列的存在。滴答声。尝试使用立克次体和弗朗西斯菌特异性引物进行种水平鉴定。Pan-Nairovirus引物在扇头蜱属、璃眼蜱属、硬蜱属的细胞系中扩增特异性不确定的PCR产物。卡里奥斯和鸟类。滴答声。用克里米亚-刚果出血热病毒片段特异性引物进行的进一步表征证实了细胞中不存在这种虫媒病毒。一组泛黄病毒引物在任何细胞系中均未检测到内源性病毒。透射电子显微镜检查显示许多细胞系中存在内源性呼肠孤病毒样病毒;其中仅4个细胞系使用蜱环状病毒圣克罗伊河病毒特异性引物得到阳性结果，表明可能存在其他尚未描述的“仅蜱”病毒栖息在蜱细胞系中。(C)2012年爱思唯尔有限公司。All rights reserved.
As well as being vectors of many viral, bacterial, and protozoan pathogens of medical and veterinary importance, ticks harbour a variety of microorganisms which are not known to be pathogenic for vertebrate hosts. Continuous cell lines established from ixodid and argasid ticks could be infected with such endosymbiotic bacteria and endogenous viruses, but to date very few cell lines have been examined for their presence. DNA and RNA extracted from over 50 tick cell lines deposited in the Roslin Wellcome Trust Tick Cell Biobank (http://tickcells.roslin.ac.uk) were screened for presence of bacteria and RNA viruses, respectively. Sequencing of PCR products amplified using pan-16S rRNA primers revealed the presence of DNA sequences from bacterial endosymbionts in several cell lines derived from Amblyomma and Dermacentor spp. ticks. Identification to species level was attempted using Rickettsia- and Francisella-specific primers. Pan-Nairovirus primers amplified PCR products of uncertain specificity in cell lines derived from Rhipicephalus, Hyalomma, Ixodes. Carios, and Ornithodoros spp. ticks. Further characterisation attempted with primers specific for Crimean-Congo haemorrhagic fever virus segments confirmed the absence of this arbovirus in the cells. A set of pan-Flavivirus primers did not detect endogenous viruses in any of the cell lines. Transmission electron microscopy revealed the presence of endogenous reovirus-like viruses in many of the cell lines; only 4 of these lines gave positive results with primers specific for the tick Orbivirus St Croix River virus, indicating that there may be additional, as yet undescribed 'tick-only' viruses inhabiting tick cell lines. (C) 2012 Elsevier GmbH. All rights reserved.