An integrated acoustic and dielectrophoretic particle manipulation in a microfluidic device for particle wash and separation fabricated by mechanical machining
An integrated acoustic and dielectrophoretic particle manipulation in a microfluidic device for particle wash and separation fabricated by mechanical machining
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DOI：
10.1063/1.4940431
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发表时间：
2016-01-01
期刊：
影响因子：
3.2
通讯作者：
Buyukkocak, Suleyman
中科院分区：
文献类型：
--
作者：
Cetin, Barbaros;Ozer, Mehmet Bulent;Buyukkocak, Suleyman
在这项研究中，声电泳和介电泳是利用在一个集成的方式，联合收割机结合在一个聚二甲基硅氧烷（PDMS）芯片上的两个不同的操作顺序的方式，即，颗粒洗涤（缓冲液交换）和颗粒分离。在洗涤步骤中，颗粒用具有低电导率的缓冲溶液洗涤以用于基于介电泳的分离，以避免焦耳加热的不利影响。由压电材料产生的声波被用于清洗，这沿着通道的整个宽度产生驻波。机电声耦合三维多物理场分析表明，压电致动器的位置和方向是成功操作的关键。独特的模具设计用于压电材料和3D侧壁电极的精确对准，以实现高度可重复的制造。为了实现集成中声电泳和介电电泳的通量匹配，使用了3D侧壁电极。该集成器件采用PDMS模压成型。使用高精度机械加工制造集成器件的模具。通过独特的模具设计，两种压电材料和3D侧壁电极的放置在模制过程中完成。结果表明，该装置可以成功地处理洗涤和介电泳分离。(C)2016 AIP Publishing LLC.
In this study, acoustophoresis and dielectrophoresis are utilized in an integrated manner to combine the two different operations on a single polydimethylsiloxane (PDMS) chip in sequential manner, namely, particle wash (buffer exchange) and particle separation. In the washing step, particles are washed with buffer solution with low conductivity for dielectrophoretic based separation to avoid the adverse effects of Joule heating. Acoustic waves generated by piezoelectric material are utilized for washing, which creates standing waves along the whole width of the channel. Coupled electro-mechanical acoustic 3D multi-physics analysis showed that the position and orientation of the piezoelectric actuators are critical for successful operation. A unique mold is designed for the precise alignment of the piezoelectric materials and 3D side-wall electrodes for a highly reproducible fabrication. To achieve the throughput matching of acoustophoresis and dielectrophoresis in the integration, 3D side-wall electrodes are used. The integrated device is fabricated by PDMS molding. The mold of the integrated device is fabricated using high-precision mechanical machining. With a unique mold design, the placements of the two piezoelectric materials and the 3D sidewall electrodes are accomplished during the molding process. It is shown that the proposed device can handle the wash and dielectrophoretic separation successfully. (C) 2016 AIP Publishing LLC.