Glycoconjugated chitosan stabilized iron oxide nanoparticles as a multifunctional nanoprobe
Glycoconjugated chitosan stabilized iron oxide nanoparticles as a multifunctional nanoprobe
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DOI：
10.1016/j.msec.2009.01.005
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发表时间：
2009-06-01
期刊：
影响因子：
--
通讯作者：
Do Ghim, Han
中科院分区：
文献类型：
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作者：
Bahadur K C, Remant;Lee, So Min;Do Ghim, Han
用功能聚合物对纳米氧化铁进行表面修饰，可以制备多功能纳米探针。研究了糖化壳聚糖(GC)稳定IOPS(GC-IOPS)的制备方法。将乳糖酸(LA)直接偶联到壳聚糖上，制备了GC。随后将GC接枝到IOPS表面以增强胶体稳定性。用X射线衍射仪、傅里叶变换红外光谱仪、透射电子显微镜、动态光散射、电泳光散射、超导量子干涉仪等测试手段对纳米粒子的性质进行了研究。GC-IOPS的FT-IR和XRD分析表明，壳聚糖的主链和侧链官能度以及IOPS的物相纯度在偶联过程中保持不变。透射电子显微镜观察表明，GC-IOPS呈球形(8-10 nm)，但其在醋酸酯缓冲液(pH 7.4)中的分散性和稳定性随壳聚糖取代度(DS)的增加而线性增加。GC-IOPS的比磁化强度随DS的变化在19.50-41.56emu/g之间变化，胶体稳定性和比磁化强度的变化表明DS可以改变IOPS的分散程度和磁性。GC-IOPS的优点是能够实现均匀的纳米颗粒分布和特定的表面功能以用于生物结合。这些特性使GC-IOPS成为生物医学研究和临床诊断的潜在候选者。(C)2009爱思唯尔B.V.保留所有权利。
Surface modification of iron oxide nanoparticles (IOPs) with functional polymer can be used for the preparation of multifunction nanoprobes. The present study dealt with the preparation of glycoconjugated chitosan (GC) stabilized IOPs (GC-IOPs). GC was prepared by direct coupling of lactobionic acid (LA) on chitosan. GC was subsequently grafted onto the surface of IOPs to enhance colloid stability. X-ray diffraction (XRD), Fourier transform infrared spectrometer (FT-IR), transmission electron microscopy (TEM), dynamic light scattering (DLS), electrophoretic light scattering (ELS) and superconducting quantum interference device (SQUID) measurements were performed to investigate the properties of nanoparticles. FT-IR and XRD analysis of GC-IOPs showed that backbone and side chain functionality of chitosan and phase purity of IOPs remained intact during conjugation. TEM observations revealed that GC-IOPs were spherical (8-10 nm) but the dispersibility and stability in acetated buffer (pH 7.4) linearly increased with degree of substitution (DS) of chitosan. The specific magnetization of GC-IOPs was varied with DS from 19.50 to 41.56 emu/g. This variation in colloid stability and specific magnetization suggests that DS can be varied to tailor the degree of dispersion and magnetic properties of IOPs. The advantage of GC-IOPS is the ability to achieve a homogeneous nanosize particle distribution and specific surface functionality for bioconjugation. These characteristics make the GC-IOPs a potential candidate for biomedical research and clinical diagnosis. (C) 2009 Elsevier B.V. All rights reserved.