Vitamin E supplementation and in vivo immune response in healthy elderly subjects - A randomized controlled trial
Vitamin E supplementation and in vivo immune response in healthy elderly subjects - A randomized controlled trial
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DOI：
10.1001/jama.277.17.1380
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发表时间：
1997-05-07
影响因子：
120.7
通讯作者：
Stollar, BD
中科院分区：
文献类型：
--
作者：
Meydani, SN;Meydani, M;Stollar, BD
客观。-确定长期补充维生素E是否能增强健康老年受试者体内细胞介导免疫的临床相关指标。随机、双盲、安慰剂对照干预研究。设置和参与者。共有88名自由生活的健康受试者，年龄至少为65岁。受试者被随机分配到安慰剂组或摄入60，200或800 mg/d维生素E的组，持续235天。迟发型超敏反应皮肤反应（DTH）;抗体反应，乙型肝炎B，破伤风和白喉，肺炎球菌疫苗;和自身抗体的DNA和甲状腺球蛋白进行了评估之前和之后的接种。补充维生素E 4个月改善了健康老年人细胞介导免疫的某些临床相关指标。与安慰剂组（分别为17%和S倍）、60 mg/d组（分别为41%和3倍）和800 mg/d组（分别为49%和2.5倍）相比，摄入200 mg/d维生素E的受试者DTH增加65%，乙型肝炎B抗体滴度增加6倍。200 mg/d组的破伤风疫苗抗体滴度也显著增加。补充后血清α-生育酚（维生素E）浓度（>48.4 μ mol/l [2.08 mg/dL]）处于上三分位的受试者对B型肝炎和DTH的抗体应答较高。补充维生素E对白喉抗体滴度没有影响，也不影响免疫球蛋白水平或T和B细胞水平。补充维生素E对自身抗体水平无显著影响。我们的研究结果表明，维生素E的水平高于目前推荐的增强某些临床相关的体内指标的T细胞介导的功能在健康的老年人。补充维生素E没有观察到任何不良影响。
Objective.-To determine whether long-term supplementation with vitamin E enhances in vivo, clinically relevant measures of cell-mediated immunity in healthy elderly subjects.Design.-Randomized, double-blind, placebo-controlled intervention study.Setting and Participants.-A total of 88 free-living, healthy subjects at least 65 years of age.Intervention.-Subjects were randomly assigned to a placebo group or to groups consuming 60, 200, or 800 mg/d of vitamin E for 235 days.Main Outcome Measures.-Delayed-type hypersensitivity skin response (DTH); antibody response to hepatitis B, tetanus and diphtheria, and pneumococcal vaccines; and autoantibodies to DNA and thyroglobulin were assessed before and after supplementation.Results.-Supplementation with vitamin E for 4 months improved certain clinically relevant indexes of cell-mediated immunity in healthy elderly. Subjects consuming 200 mg/d of vitamin E had a 65% increase in DTH and a 6-fold increase in antibody titer to hepatitis B compared with placebo (17% and S-fold, respectively), 60-mg/d (41% and 3-fold, respectively), and 800-mg/d (49% and 2.5-fold, respectively) groups. The 200-mg/d group also had a significant increase in antibody titer to tetanus vaccine. Subjects in the upper tertile of serum a-tocopherol (vitamin E) concentration (>48.4 mu mol/l [2.08 mg/dL]) after supplementation had higher antibody response to hepatitis B and DTH. Vitamin E supplementation had no effect on antibody titer to diphtheria and did not affect immunoglobulin levels or levels of T and B cells. No significant effect of vitamin E supplementation on autoantibody levels was observed.Conclusions.-Our results indicate that a level of vitamin E greater than currently recommended enhances certain clinically relevant in vivo indexes of T-cell-mediated function in healthy elderly persons. No adverse effects were observed with vitamin E supplementation.