Iron Absorption in the Rat: The Search for Possible Intestinal Mucosal Carriers
Iron Absorption in the Rat: The Search for Possible Intestinal Mucosal Carriers
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大鼠的铁吸收：寻找可能的肠粘膜载体
DOI：
10.1111/j.1365-2141.1976.tb00194.x
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发表时间：
1976
影响因子：
6.5
通讯作者：
U. Mack
中科院分区：
文献类型：
--
作者：
J. Halliday;L. Powell;U. Mack
总结。研究了以前在大鼠肠粘膜细胞中检测到的四种含铁组份的生物学相关性。用Sepharose6B柱层析得到的这些组分，在体内外与59Fe孵育后，观察了铁在这些组分中的分布。此外，还研究了苯巴比妥、放线菌胺、缺铁和铁负荷对四种粘膜细胞铁摄取和分布的影响。组分I中的铁主要结合在细胞膜颗粒上。组份II为铁蛋白。组分III含有部分转铁蛋白和一种分子量与转铁蛋白相似的蛋白质，但不能被抗转铁蛋白抗血清沉淀。体内和体外研究的数量差异结合‘Chaser’实验的结果表明，除了铁蛋白外，至少有两个组分(I和III)参与了粘膜细胞对铁的吸收过程。
SUMMARY. The biological relevance of four iron‐containing fractions previously detected in rat intestinal mucosal cells has been studied. The distribution of iron in these fractions obtained by chromatography on Sepharose 6B has been examined after in vivo and in vitro incubation of mucosal cells with 59Fe. In addition, the effects of phenobarbitone, cycloheximide, iron‐deficiency and iron‐loading on the uptake and distribution of iron within the four mucosal cell fractions was studied. The iron in fraction I was mostly bound to intracellular membrane particles. Fraction II was shown to be ferritin. Fraction III contained some transferrin and also a protein of molecular weight similar to transferrin but which was not precipitable by antitransferrin antiserum. Quantitative differences between the in vivo and in vitro studies combined with the results of ‘chaser’experiments suggested that, in addition to ferritin, at least two of the fractions (I and III) were involved in the process of iron absorption by the mucosal cell.