EST-based profiling and comparison of gene expression in the silkworm fat body during metamorphosis
EST-based profiling and comparison of gene expression in the silkworm fat body during metamorphosis
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DOI：
10.1002/arch.20090
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发表时间：
2006-01-01
影响因子：
2.2
通讯作者：
Xiang, ZH
中科院分区：
文献类型：
--
作者：
Cheng, DJ;Xia, QY;Xiang, ZH
脂肪体在蚕一生的新陈代谢和生长维持中发挥着关键作用，因此代表了研究发育和变态的模型组织。对来自幼虫和蛹脂肪体 cDNA 文库的 18,480 个 EST 进行分析，可以表征变态过程中脂肪体中的基因表达模式。通过 Phrap 组装，生成了 6,814 个估计转录单位 (TU)，其中 2,673 个包含 EST 总数的 60%，代表 1,366 个独特的已知基因。这些基因根据其已知或假定的功能被分为 14 类，其中大多数被发现与新陈代谢和细胞防御有关。对表达谱的进一步比较分析表明，幼虫脂肪体中的基因表达模式与蛹脂肪体中的基因表达模式存在显着差异。由此，我们鉴定了可能与变质事件（例如特定组织的形成或破坏）相关的基因子集，并同时使用 RT-PCR 检查确认了几个已鉴定基因的阶段特异性表达模式。这项工作将为研究与脂肪体发育变化和蚕变态相关的调控机制提供宝贵的资源。
The fat body plays key roles in metabolism and sustenance of growth throughout the life of a silkworm, and thus represents a model tissue for studying development and metamorphosis. Analysis of 18,480 ESTs derived from larval and pupal fat body cDNA libraries allowed characterization of gene expression patterns in the fat body during metamorphosis. By Phrap assembling, 6,814 estimated transcription units (TUs) were generated, 2,673 of which contained 60% of the total ESTs and represented 1,366 distinctive known genes. These genes were classified into 14 categories based on their known or putative functions, and most were found to be involved in metabolism and cell defense. Further comparative analysis of the expression profiles revealed that the gene expression patterns in the larval fat body strikingly differed from those in the pupal fat body. From this, we identified a subset of genes potentially associated with metamorphic events such as the formation or destruction of specific tissues, and simultaneously confirmed the stage-specific expression patterns of several identified genes using RT-PCR examination. This work will provide a valuable resource for studying regulatory mechanisms associated with fat body developmental changes and silkworm metamorphosis.